Apoptosis induction and cell cycle perturbation in human hepatoma Hep G2 cells by 10-hydroxycamptothecin
文献类型：期刊论文
| 作者 | Zhang, XW; Qing, C; Xu, B
|
| 刊名 | ANTI-CANCER DRUGS
 |
| 出版日期 | 1999-07 |
| 卷号 | 10期号:6页码:569-576 |
| 关键词 | 10-hydroxycamptothecin apoptosis cell cycle flow cytometry Hep G2 cells |
| ISSN号 | 0959-4973 |
| DOI | 10.1097/00001813-199907000-00009 |
| 文献子类 | Article |
| 英文摘要 | 10-Hydroxycamptothecin (HCPT), a DNA topoisomerase I inhibitor, is an antitumor alkaloid isolated from a Chinese tree, Camptotheca acuminata, and exhibits a remarkable antihepatoma effect. We studied HCPT to determine whether or not its anti-hepatoma activity occurs through apoptosis induction and cell cycle disturbance using the MTT method, DAPI staining, agarose gel electrophoresis and flow cytometric analysis. The results showed that HCPT inhibited proliferation of human hepatoma Hep G2, Bel-7402 and Bel-7404 cells at an optimal concentration of 0.1 mu g/ml. This growth inhibition was dose and time dependent, and was accompanied by evidence of apoptotic changes and cell cycle perturbation in Hep G2 cells. Chromatin condensation and nuclear fragmentation were observed in Hep G2 cells by fluorescence microscopy. Agarose gel electrophoresis showed internucleosomal DNA fragmentation ('ladder pattern') of Hep G2 cells following treatment with HCPT, in a concentration- and time-dependent manner. Flow cytometry showed that HCPT induced a massive hypodiploid cell population and arrested cells in G(2)/M phase (at low dose) or in S phase (at high dose) in Hep G2 cells. The results of this study suggest that the anti-hepatoma effect of HCPT may result from apoptosis induction and cell cycle disturbance. [(C) 1999 Lippincott Williams & Wilkins.]. |
| WOS关键词 | DNA TOPOISOMERASE-I ; CAMPTOTHECIN ; CARCINOMA ; CHINA |
| WOS研究方向 | Oncology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000081902200009 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274713]  |
| 专题 | 院士及顾问专家 药物安全性评价中心 国家新药筛选中心 |
| 通讯作者 | Xu, B |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XW,Qing, C,Xu, B. Apoptosis induction and cell cycle perturbation in human hepatoma Hep G2 cells by 10-hydroxycamptothecin[J]. ANTI-CANCER DRUGS,1999,10(6):569-576. |
| APA | Zhang, XW,Qing, C,&Xu, B.(1999).Apoptosis induction and cell cycle perturbation in human hepatoma Hep G2 cells by 10-hydroxycamptothecin.ANTI-CANCER DRUGS,10(6),569-576. |
| MLA | Zhang, XW,et al."Apoptosis induction and cell cycle perturbation in human hepatoma Hep G2 cells by 10-hydroxycamptothecin".ANTI-CANCER DRUGS 10.6(1999):569-576. |
入库方式： OAI收割
来源：上海药物研究所
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