Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications
文献类型：期刊论文
| 作者 | Lou, Ruyun1,2; Yu, Weiting2; Song, Yizhe1,2; Ren, Ying1,2; Zheng, Huizhen1,2; Guo, Xin2; Lin, Yunfei3; Pan, Guoyu3 ; Wang, Xiuli4; Ma, Xiaojun2
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| 刊名 | CARBOHYDRATE POLYMERS
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| 出版日期 | 2017-01-02 |
| 卷号 | 155页码:456-465 |
| 关键词 | Galactosylated alginate Hydroxyl groups Microcapsules Primary hepatocytes Biological artificial liver support systems |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2016.08.098 |
| 文献子类 | Article |
| 英文摘要 | Galactose moieties are covalently coupled with sodium alginate to enhance liver-specific functions in microcapsules owing to the specific interaction between the galactose moieties and the asialoglycoprotein receptors (ASGPRs) of hepatocytes. In this study, galactosylated alginate (L-NH2-OH-alginate) based microcapsules with desirable stability and a suitable 3D microenvironment are designed and fabricated for primary hepatocyte applications. The designed L-NH2-OH-alginate is fabricated via the application of ethylenediamine grafted lactobionic acid (L-NH2) onto the hydroxyl groups of sodium alginate so that the negatively charged carboxyl groups intact in L-NH2-OH-alginate can effectively bond with Ca2+ to form a stable three-dimensional gel network; a subsequent reaction with polycations forms a stable membrane of microcapsules. As a result, L-NH2-OH-alginate based microcapsules exhibit an excellent mechanical stability. Moreover, with a higher degree of substitution in L-NH2-OH-alginate (DS 0.41), the hepatocytes entrapped in L-NH2-OH-alginate microcapsules exhibit better viability and well-maintained liver-specific functions. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS关键词 | MICROENCAPSULATED HEPATOCYTES ; MECHANICAL STABILITY ; EXTRACELLULAR-MATRIX ; BIOARTIFICIAL LIVER ; CULTURE ; CHITOSAN ; RAT ; ENCAPSULATION ; OPTIMIZATION ; SUBSTRATUM |
| 资助项目 | National Basic Research Program of China[2012CB720801] ; Scientific and Technological projects of Guangdong Province[2013B091100001] ; National Natural Science Foundation of China[81173125] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000386402600050 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275687]  |
| 专题 | 药物安全性评价中心 |
| 通讯作者 | Yu, Weiting; Wang, Xiuli |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai, Peoples R China 4.Dalian Med Univ, Dept Histol & Embryol, Coll Basic Med Sci, Dalian 116044, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lou, Ruyun,Yu, Weiting,Song, Yizhe,et al. Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications[J]. CARBOHYDRATE POLYMERS,2017,155:456-465. |
| APA | Lou, Ruyun.,Yu, Weiting.,Song, Yizhe.,Ren, Ying.,Zheng, Huizhen.,...&Ma, Xiaojun.(2017).Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications.CARBOHYDRATE POLYMERS,155,456-465. |
| MLA | Lou, Ruyun,et al."Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications".CARBOHYDRATE POLYMERS 155(2017):456-465. |
入库方式： OAI收割
来源：上海药物研究所
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