Identification of Two Additional Susceptibility Loci for Inflammatory Bowel Disease in a Chinese Population
文献类型：期刊论文
| 作者 | Lan, Xiucai1,5,6; Lan, Xiuhua2; Chang, Ying1,5,6; Zhang, Xiaomin1,5,6; Liu, Jing1,5,6; Vikash, Vikash4; Wang, Wei3; Huang, Meifang1,5,6; Wang, Xiaobing1,5,6; Zhou, Feng1,5,6 |
| 刊名 | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 41期号:5页码:2077-2090 |
| 关键词 | ZMIZ1 TP53 TNFSF15 TL1A IBD Polymorphism |
| ISSN号 | 1015-8987 |
| DOI | 10.1159/000475439 |
| 文献子类 | Article |
| 英文摘要 | Background/Aims: To investigate the associations between the rs1250569 (zinc f.inger MIZ-type containing 1, ZMIZ1), rs1042522 (tumour protein p53, TP53), and rs10114470 (tumour necrosis factor-like cytokine 1A, TL1A) polymorphisms and the development of inflammatory bowel disease (IBD) in a Chinese (Han) population. We analysed the expression of genes that predispose patients to Crohn's disease (CD) and ulcerative colitis (UC). Methods: A total of 381 IBD patients and 517 healthy controls were recruited into our study. Polymorphisms at the three loci were genotyped using polymerase chain reaction-ligation detection reactions (PCR-LDR). Genotype-phenotype correlations were analysed. Blood and gut samples were obtained and analysed using quantitative real-time PCR (qRT-PCR), western blot analysis, and immunohistochemistry to investigate the mRNA and protein levels and in situ expression of genes found to predispose patients to IBD. Furthermore, the expression of susceptible genes was further verified using a mouse dextran sulphate sodium (DSS)-induced acute colitis model. Results: No significant association was detected between rs1250569 and rs1042522 genotypes and CD or UC susceptibility. However, the frequency of allele A of rs1250569 was much higher in CD patients than that in healthy controls (55.03% vs. 48.48%, respectively; p = 0.044). The mutation rates at rs10114470 were dramatically lower at both the genotype and allele level in patients than those in healthy controls (p = 0.002 at both the genotype and allele level). Additionally, increased ZMIZ1 and TL1A levels were detected in intestinal samples obtained from both IBD patients and DSS-treated mice. Conclusion: rs1250569 (ZMIZ1) and rs10114470 (TL1A) are two novel loci that indicate susceptibility to IBD in Han-Chinese patients. Consistent with previous studies, TL1A expression levels were higher in Chinese Han IBD patients and DSS-treated mice. Most importantly, we found that ZMIZ1 expression was markedly higher in both IBD patients and mice with experimentally induced colitis, suggesting that ZMIZ1 plays important roles in the pathogenesis of IBD. (C) 2017 The Author(s). Published by S. Karger AG, Basel. |
| WOS关键词 | GENOME-WIDE ASSOCIATION ; CROHNS-DISEASE ; ULCERATIVE-COLITIS ; TGF-BETA ; GENETIC SUSCEPTIBILITY ; IMMUNE-RESPONSES ; PROTEIN HZIMP10 ; POLYMORPHISMS ; CANCER ; VARIANTS |
| 资助项目 | National Natural Science Foundation of China[81270467] ; National Natural Science Foundation of China[81470823] |
| WOS研究方向 | Cell Biology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000404173800030 |
| 出版者 | KARGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275709]  |
| 专题 | 药物安全性评价中心 |
| 通讯作者 | Chen, Liping; Zhao, Qiu |
| 作者单位 | 1.Hubei Clin Ctr, Wuhan, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Pathol Dept, Shanghai, Peoples R China; 3.Zhongshan Univ, Affiliated Hosp 6, Dept Gastroenterol, Guangzhou, Guangdong, Peoples R China 4.Wuhan Univ, Renmin Hosp, Dept Gastroenterol, Wuhan, Hubei, Peoples R China; 5.Wuhan Univ, Zhongnan Hosp, Dept Gastroenterol, Wuhan, Peoples R China; 6.Key Lab Intestinal & Colorectal Dis, Wuhan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lan, Xiucai,Lan, Xiuhua,Chang, Ying,et al. Identification of Two Additional Susceptibility Loci for Inflammatory Bowel Disease in a Chinese Population[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2017,41(5):2077-2090. |
| APA | Lan, Xiucai.,Lan, Xiuhua.,Chang, Ying.,Zhang, Xiaomin.,Liu, Jing.,...&Zhao, Qiu.(2017).Identification of Two Additional Susceptibility Loci for Inflammatory Bowel Disease in a Chinese Population.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,41(5),2077-2090. |
| MLA | Lan, Xiucai,et al."Identification of Two Additional Susceptibility Loci for Inflammatory Bowel Disease in a Chinese Population".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 41.5(2017):2077-2090. |
入库方式： OAI收割
来源：上海药物研究所
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