CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS
文献类型：期刊论文
| 作者 | Zhang, Lijun1; Hua, Qiuhong1; Tang, Kaiyi1; Shi, Changjie1; Xie, Xin1,2 ; Zhang, Ru1
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| 刊名 | NEUROSCIENCE
 |
| 出版日期 | 2016-11-19 |
| 卷号 | 337页码:88-97 |
| 关键词 | human embryonic stem cell neural stem/precursor cell neural differentiation chemokine receptor CXCR4 SDF-1 |
| ISSN号 | 0306-4522 |
| DOI | 10.1016/j.neuroscience.2016.09.001 |
| 文献子类 | Article |
| 英文摘要 | G protein-coupled receptors (OGPCRs) are involved in many fundamental cellular responses such as growth, death, movement, transcription and excitation. Their roles in human stem cell neural specialization are not well understood. In this study, we aimed to identify GPCRs that may play a role in the differentiation of human embryonic stem cells (OhESCs) to neural stem cells (ONSCs). Using a feeder-free hESC neural differentiation protocol, we found that the expression of several chemokine receptors changed dramatically during the hESC/NSC transition. Especially, the expression of CXCR4 increased approximately 50 folds in NSCs compared to the original hESCs. CXCR4 agonist SDF-1 promoted, whereas the antagonist AMD3100 delayed the neural induction process. In consistence with antagonizing CXCR4, knockdown of CXCR4 in hESCs also blocked the neural induction and cells with reduced CXCR4 were rarely positive for Nestin and Sox1-staining. Taken together, our results suggest that CXCR4 is involved in the neural induction process of hESC and it might be considered as a target to facilitate NSC production from hESCs in regenerative medicine. (C) 2016 The Authors. Published by Elsevier Ltd on behalf of IBRO. This is an open access article under the CCBY-NC-ND license. |
| WOS关键词 | PROTEIN-COUPLED RECEPTORS ; CHEMOKINE RECEPTOR ; PROGENITOR CELLS ; ADULT NEUROGENESIS ; SIGNALING PATHWAY ; DENTATE GYRUS ; IN-VITRO ; MIGRATION ; PROLIFERATION ; CXCL12 |
| 资助项目 | Ministry of Science and Technology of China[2014CB965002] ; Ministry of Science and Technology of China[2015CB964503] ; Science and Technology Commission of Shanghai Municipality, China[15JC1400202] ; Chinese Academy of Sciences, China[XDA01040301] ; National Natural Science Foundation of China[81425024] ; National Natural Science Foundation of China[31371511] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000386337600008 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275803]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Xie, Xin; Zhang, Ru |
| 作者单位 | 1.Tongji Univ, Sch Life Sci & Technol, Collaborat Innovat Ctr Brain Sci, Shanghai Key Lab Signaling & Dis Res,Lab Receptor, Shanghai 200092, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lijun,Hua, Qiuhong,Tang, Kaiyi,et al. CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS[J]. NEUROSCIENCE,2016,337:88-97. |
| APA | Zhang, Lijun,Hua, Qiuhong,Tang, Kaiyi,Shi, Changjie,Xie, Xin,&Zhang, Ru.(2016).CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS.NEUROSCIENCE,337,88-97. |
| MLA | Zhang, Lijun,et al."CXCR4 ACTIVATION PROMOTES DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO NEURAL STEM CELLS".NEUROSCIENCE 337(2016):88-97. |
入库方式： OAI收割
来源：上海药物研究所
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