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DWARF14 is a non-canonical hormone receptor for strigolactone
文献类型：期刊论文
| 作者 | Yao, Ruifeng6,7; Ming, Zhenhua8,9; Yan, Liming8,9; Li, Suhua6,7; Wang, Fei6,7; Ma, Sui6,7; Yu, Caiting6,7; Yang, Mai6,7; Chen, Li6,7; Chen, Linhai1
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| 刊名 | NATURE
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| 出版日期 | 2016-08-25 |
| 卷号 | 536期号:7617页码:469-+ |
| ISSN号 | 0028-0836 |
| DOI | 10.1038/nature19073 |
| 文献子类 | Article |
| 英文摘要 | Classical hormone receptors reversibly and non-covalently bind active hormone molecules, which are generated by biosynthetic enzymes, to trigger signal transduction. The alpha/beta hydrolase DWARF14 (D14), which hydrolyses the plant branching hormone strigolactone and interacts with the F-box protein D3/MAX2, is probably involved in strigolactone detection(1-3). However, the active form of strigolactone has yet to be identified and it is unclear which protein directly binds the active form of strigolactone, and in which manner, to act as the genuine strigolactone receptor. Here we report the crystal structure of the strigolactone-induced AtD14-D3-ASK1 complex, reveal that Arabidopsis thaliana (At) D14 undergoes an open-to-closed state transition to trigger strigolactone signalling, and demonstrate that strigolactone is hydrolysed into a covalently linked intermediate molecule (CLIM) to initiate a conformational change of AtD14 to facilitate interaction with D3. Notably, analyses of a highly branched Arabidopsis mutant d14-5 show that the AtD14(G158E) mutant maintains enzyme activity to hydrolyse strigolactone, but fails to efficiently interact with D3/MAX2 and loses the ability to act as a receptor that triggers strigolactone signalling in planta. These findings uncover a mechanism underlying the allosteric activation of AtD14 by strigolactone hydrolysis into CLIM, and define AtD14 as a non-canonical hormone receptor with dual functions to generate and sense the active form of strigolactone. |
| WOS关键词 | ARABIDOPSIS-THALIANA ; STRUCTURAL BASIS ; PERCEPTION ; RICE ; DEGRADATION ; INHIBITION ; KARRIKIN ; RECOGNITION ; MECHANISM ; REPRESSOR |
| 资助项目 | National Natural Science Foundation of China[91417302] ; National Natural Science Foundation of China[81322023] ; National Natural Science Foundation of China[31421001] ; National Natural Science Foundation of China[91335204] ; Ministry of Science and Technology[2013CB911100] ; Ministry of Science and Technology[2016YFA0500500] ; Ministry of Agriculture[2014ZX08011006] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000382646600044 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275923]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Rao, Zihe; Lou, Zhiyong; Xie, Daoxin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; 3.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China; 4.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China; 5.Tsinghua Univ, Sch Pharmaceut Sci, Beijing 100084, Peoples R China 6.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China; 7.Tsinghua Univ, Sch Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China; 8.Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China; 9.Tsinghua Univ, Collaborat Innovat Ctr Biotherapy, Beijing 100084, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yao, Ruifeng,Ming, Zhenhua,Yan, Liming,et al. DWARF14 is a non-canonical hormone receptor for strigolactone[J]. NATURE,2016,536(7617):469-+. |
| APA | Yao, Ruifeng.,Ming, Zhenhua.,Yan, Liming.,Li, Suhua.,Wang, Fei.,...&Xie, Daoxin.(2016).DWARF14 is a non-canonical hormone receptor for strigolactone.NATURE,536(7617),469-+. |
| MLA | Yao, Ruifeng,et al."DWARF14 is a non-canonical hormone receptor for strigolactone".NATURE 536.7617(2016):469-+. |
入库方式： OAI收割
来源：上海药物研究所
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