Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails
文献类型：期刊论文
| 作者 | Fu, Yanbin1; Huang, Chenwen1; Xu, Xinxiu2; Gu, Haifeng2 ; Ye, Youqiong1; Jiang, Cizhong1; Qiu, Zilong3; Xie, Xin1,2
|
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2015-09 |
| 卷号 | 25期号:9页码:1013-1024 |
| 关键词 | transdifferentiation cardiac reprogramming cardiomyocyte fibroblast chemical cocktail small molecule compounds |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2015.99 |
| 文献子类 | Article |
| 英文摘要 | The direct conversion, or transdifferentiation, of non-cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provides promising approaches for cardiac regeneration. However, genetic manipulations raise safety concerns and are thus not desirable in most clinical applications. The discovery of full chemically induced pluripotent stem cells suggest the possibility of replacing transcription factors with chemical cocktails. Here, we report the generation of automatically beating cardiomyocyte-like cells from mouse fibroblasts using only chemical cocktails. These chemical-induced cardiomyocyte-like cells (CiCMs) express cardiomyocyte-specific markers, exhibit sarcomeric organization, and possess typical cardiac calcium flux and electrophysiological features. Genetic lineage tracing confirms the fibroblast origin of these CiCMs. Further studies show the generation of CiCMs passes through a cardiac progenitor stage instead of a pluripotent stage. Bypassing the use of viral-derived factors, this proof of concept study lays a foundation for in vivo cardiac transdifferentiation with pharmacological agents and possibly safer treatment of heart failure. |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; ISCHEMIC-HEART-DISEASE ; IN-VIVO ; CARDIAC FIBROBLASTS ; PROGENITOR CELLS ; SOMATIC-CELLS ; ADULT HEART ; TGF-BETA ; DIFFERENTIATION ; REPAIR |
| 资助项目 | Chinese Academy of Sciences[XDA01040301] ; Ministry of Science and Technology of China[2015CB964503] ; National Natural Science Foundation of China[81425024] ; National Natural Science Foundation of China[31371511] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5516193 |
| WOS记录号 | WOS:000360765600006 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276420]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Xie, Xin |
| 作者单位 | 1.Tongji Univ, Shanghai Key Lab Signaling & Dis Res, Sch Life Sci & Technol, Lab Receptor Based Biomed, Shanghai 200092, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Inst Neurosci,Key Lab Primate Neurobiol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Yanbin,Huang, Chenwen,Xu, Xinxiu,et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails[J]. CELL RESEARCH,2015,25(9):1013-1024. |
| APA | Fu, Yanbin.,Huang, Chenwen.,Xu, Xinxiu.,Gu, Haifeng.,Ye, Youqiong.,...&Xie, Xin.(2015).Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails.CELL RESEARCH,25(9),1013-1024. |
| MLA | Fu, Yanbin,et al."Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails".CELL RESEARCH 25.9(2015):1013-1024. |
入库方式： OAI收割
来源：上海药物研究所
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