Establishment and Application of a High-throughput-screening Model for Medium-chain Fatty Acid-sensing Receptor GPR84
文献类型：期刊论文
| 作者 | Zhang Qing1 ; Yang Hui2; Li Jia1,2; Xie Xin2; Li Jing2
|
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2015-04 |
| 卷号 | 42期号:4页码:337-347 |
| 关键词 | GPR84 stably expressing calcium mobilization assay high-throughput screening |
| ISSN号 | 1000-3282 |
| 文献子类 | Article |
| 英文摘要 | G protein coupled receptor 84(GPR84) is medium-chain fatty acid-sensing receptor(C9 similar to C14). It plays an important role in metabolism of fatty acid and immune response, and it's also deeply involved in inflammatory immune diseases such as multiple sclerosis and endotoxemia. It may provide an effective method to treat these diseases by finding GPR84 ligands. It's important to construct a cell line stably expressing human GPR84 for screening its ligands and researching diseases. In this research, we transfected plasmids encoding GPR84 and G alpha 16 proteins into HEK293 cells. After selection with antibiotics, we picked out monoclones which stably expresses GPR84 to form cell lines. The permanent expression of exogenous GPR84 proteins in HEK293 cells was detected by RT-PCR, immunofluorescence staining, calcium mobilization and cAMP assay. Western blot and FACS were conducted to examine the activity of GPR84. Above all the results demonstrate that GPR84 receptor has excellent biology activity. Based on cell lines stably expressing GPR84, we conducted high-throughput assay for the screening of GPR84 ligands and found new GPR84 antagonists. We can further research on the biological functions of GPR84 and immune diseases targeted GPR84 with these antagonists. |
| WOS关键词 | PROTEIN-COUPLED RECEPTORS ; GPR120 ; DISCOVERY ; ASSAY |
| 资助项目 | National Science and Technology Major Project[2012ZX09301001 -005] ; National Science and Technology Major Project[2013ZX09507001] ; National Basic Research Program of China (973 Program)[2014CB541900] ; National Natural Science Foundation of China[81202341] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5398369 |
| WOS记录号 | WOS:000353528300005 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276584]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Li Jing |
| 作者单位 | 1.E China Normal Univ, Inst Adv Interdisciplinary Res, Shanghai 200062, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Qing,Yang Hui,Li Jia,et al. Establishment and Application of a High-throughput-screening Model for Medium-chain Fatty Acid-sensing Receptor GPR84[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2015,42(4):337-347. |
| APA | Zhang Qing,Yang Hui,Li Jia,Xie Xin,&Li Jing.(2015).Establishment and Application of a High-throughput-screening Model for Medium-chain Fatty Acid-sensing Receptor GPR84.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,42(4),337-347. |
| MLA | Zhang Qing,et al."Establishment and Application of a High-throughput-screening Model for Medium-chain Fatty Acid-sensing Receptor GPR84".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 42.4(2015):337-347. |
入库方式： OAI收割
来源：上海药物研究所
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