Somatostatin receptor type 2 contributes to the self-renewal of murine embryonic stem cells
文献类型：期刊论文
| 作者 | Xu, Xin-xiu1,2; Zhang, Li-hong2; Xie, Xin1,2
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2014-08 |
| 卷号 | 35期号:8页码:1023-1030 |
| 关键词 | stem cell mouse embryonic stem cell GPCR somatostatin receptor type 2 self-renewal STAT3 leukemia inhibitory factor octreotide seglitide |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2014.51 |
| 文献子类 | Article |
| 英文摘要 | Aim: The roles of G-protein coupled receptors (GPCRs) in stem cell biology remain unclear. In this study, we aimed to identify GPCRs that might contribute to the self-renewal of mouse embryonic stem cells (mESCs). Methods: The expression levels of pluripotent genes and GPCR gene were detected in E14 mESCs using PCR array and RT-PCR. Immunofluorescent staining was used to examine the expression of pluripotent markers and the receptor translocation. Western blot analysis was used to detect phosphorylation of signal proteins. Knock-down of receptor was conducted to confirm its role in pluripotency maintenance. Results: In leukemia inhibitory factor (LIF)-free medium, mESCs lost the typical morphology of pluripotency, accompanied by markedly decreases in expression of somatostatin receptor type 2 (SSTR2), as well as the pluripotency biomarkers Oct4, Sox2, Rex1 and Nanog. Addition of the SSTR2 agonist octreotide or seglitide (0.1-30 mu mol/L) in LIF-free medium dose-dependently promoted the self-renewal of mESCs, whereas the SSTR2 antagonist 84 (0.03-3 mu mol/L) dose-dependently blocked octreotide-induced self-renewal. Knock-down of SSTR2 significantly decreased the self-renewal of mESCs even in the presence of LIF. Addition of LIF (1000 U/mL) or octreotide (1 mu mol/L) in LIF-free medium significantly increased both phosphorylation and nuclear localization of STAT3. Conclusion: The activation of SSTR2 contributes to the self-renewal of mESCs via activation of the STAT3 pathway. |
| WOS关键词 | LEUKEMIA INHIBITORY FACTOR ; PROTEIN COUPLED RECEPTORS ; DRUG DISCOVERY ; DIFFERENTIATION ; ACTIVATION ; SSTR2 ; PLURIPOTENCY ; MAINTENANCE ; EXPRESSION ; CYCLASE |
| 资助项目 | Chinese Academy of Sciences[XDA01040301] ; National Natural Science Foundation of China[31371511] ; Ministry of Science and Technology of China[2013ZX09507001] ; Ministry of Science and Technology of China[2012ZX09301001-005] ; Shanghai Commission of Science and Technology[12XD1402100] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5206237 |
| WOS记录号 | WOS:000340510400005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276958]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Zhang, Li-hong |
| 作者单位 | 1.Tongji Univ, Sch Life Sci & Technol, Lab Receptor Based Biomed, Shanghai Key Lab Signaling & Dis Res, Shanghai 200092, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xu, Xin-xiu,Zhang, Li-hong,Xie, Xin. Somatostatin receptor type 2 contributes to the self-renewal of murine embryonic stem cells[J]. ACTA PHARMACOLOGICA SINICA,2014,35(8):1023-1030. |
| APA | Xu, Xin-xiu,Zhang, Li-hong,&Xie, Xin.(2014).Somatostatin receptor type 2 contributes to the self-renewal of murine embryonic stem cells.ACTA PHARMACOLOGICA SINICA,35(8),1023-1030. |
| MLA | Xu, Xin-xiu,et al."Somatostatin receptor type 2 contributes to the self-renewal of murine embryonic stem cells".ACTA PHARMACOLOGICA SINICA 35.8(2014):1023-1030. |
入库方式： OAI收割
来源：上海药物研究所
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