Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study
文献类型：期刊论文
| 作者 | Zhang, Changlin2,3; Li, Peng2; Liu, Lianqing2; Wang, Yuechao2; Gao, Zhaobing1 ; Li, Guangyong4
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| 刊名 | IEEE-ASME TRANSACTIONS ON MECHATRONICS
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| 出版日期 | 2014-08 |
| 卷号 | 19期号:4页码:1138-1147 |
| 关键词 | Atomic force microscope (AFM) mechanosensitive (MS) ion channel planar patch clamp |
| ISSN号 | 1083-4435 |
| DOI | 10.1109/TMECH.2013.2272562 |
| 文献子类 | Article |
| 英文摘要 | Mechanosensitive ion channels play important roles for sensing and responding to the mechanical stimuli signals in living life. Here we report the development of a mechanostimulated patch-clamp system for simultaneous recording of external stimuli and acquisition of cellular physiological responses. This system integrates a custom-designed planar patch-clamp system with a robot-assisted atomic force microscope (AFM) system. The former, with a microfluidic channel, can realize not only recording electrical signals but also exchanging intracellular solution; while the latter, enhanced by robotic techniques (local scan force feedback, augmented reality vision feedback), can generate force stimuli with controllable patterns and magnitudes under the operator's real-time monitoring. To verify the performance of the developed system, we first measured the whole-cell current of the voltage-gated potassium ion channel Kv1.1 expressed on Human Embryonic Kidney (HEK293) cells and then recorded the mechanosensitive ion channel current in a mouse neuroblastoma cell line (Neuro2 A) in the whole-cell configuration during the AFM indenting on the membrane surface; finally, confirmed the ability to exchange intracellular solution by delivering propidium iodide into the captured cell through intracellular solution. The results prove the effectiveness of the system. |
| WOS关键词 | ATOMIC-FORCE MICROSCOPY ; GATED K+ CHANNELS ; MEMBRANE PATCHES ; SENSORY NEURONS ; SILICON CHIP ; ION CHANNELS ; LIVING CELLS ; WHOLE-CELL ; MECHANOTRANSDUCTION ; NANOMANIPULATION |
| 资助项目 | National Natural Science Foundation of China[61175803] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201245] ; CAS/SAFEA International Partnership Program for Creative Research Teams[00000000] ; National Science Foundation[CNS 1035563] |
| WOS研究方向 | Automation & Control Systems ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000335915800004 |
| 出版者 | IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276976]  |
| 专题 | 神经药理学研究国际科学家工作站 |
| 通讯作者 | Zhang, Changlin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, State Key Lab Robot, Shenyang Inst Automat, Shenyang 110016, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Univ Pittsburgh, Dept Elect & Comp Engn, Pittsburgh, PA 15213 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Changlin,Li, Peng,Liu, Lianqing,et al. Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study[J]. IEEE-ASME TRANSACTIONS ON MECHATRONICS,2014,19(4):1138-1147. |
| APA | Zhang, Changlin,Li, Peng,Liu, Lianqing,Wang, Yuechao,Gao, Zhaobing,&Li, Guangyong.(2014).Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study.IEEE-ASME TRANSACTIONS ON MECHATRONICS,19(4),1138-1147. |
| MLA | Zhang, Changlin,et al."Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study".IEEE-ASME TRANSACTIONS ON MECHATRONICS 19.4(2014):1138-1147. |
入库方式： OAI收割
来源：上海药物研究所
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