中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 国家新药筛选中心
Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies

文献类型：期刊论文

作者Zhao, Botao2; Yang, Dehua3,4; Jiang, Jing1; Li, Jinsong1; Fan, Chunsun5; Huang, Menggui6; Fan, Yi6; Jin, Yan7; Jin, Youxin2
刊名BMC GENOMICS
出版日期2014-06-18
卷号15
ISSN号1471-2164
DOI10.1186/1471-2164-15-488
文献子类Article
英文摘要Background: Reprogrammed cells, including induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cells (NT-ESCs), are similar in many respects to natural embryonic stem cells (ESCs). However, previous studies have demonstrated that iPSCs retain a gene expression signature that is unique from that of ESCs, including differences in microRNA (miRNA) expression, while NT-ESCs are more faithfully reprogrammed cells and have better developmental potential compared with iPSCs. Results: We focused on miRNA expression and explored the difference between ESCs and reprogrammed cells, especially ESCs and NT-ESCs. We also compared the distinct expression patterns among iPSCs, NT-ESCs and NT-iPSCs. The results demonstrated that reprogrammed cells (iPSCs and NT-ESCs) have unique miRNA expression patterns compared with ESCs. The comparison of differently reprogrammed cells (NT-ESCs, NT-iPSCs and iPSCs) suggests that several miRNAs have key roles in the distinct developmental potential of reprogrammed cells. Conclusions: Our data suggest that miRNAs play a part in the difference between ESCs and reprogrammed cells, as well as between MEFs and pluripotent cells. The variation of miRNA expression in reprogrammed cells derived using different reprogramming strategies suggests different characteristics induced by nuclear transfer and iPSC generation, as well as different developmental potential among NT-ESCs, iPSCs and NT-iPSCs.
WOS关键词NUCLEAR TRANSFER ; HUMAN FIBROBLASTS ; MOUSE ; MICRORNAS ; MIR-302 ; IPS ; BLASTOCYSTS ; INDUCTION ; MIRBASE ; TARGETS
资助项目National Key Research and Development Program of China[2011CB811304] ; National Natural Science Foundation of China[31170750] ; National Natural Science Foundation for Young Scholar[31100570] ; Shanghai Municipal Education Commission[12YZ032] ; Shanghai University[00000000] ; Natural Science Foundation of Shanghai City[12ZR1406900] ; State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Institutes for Biological Sciences, Chinese Academy of Sciences[00000000]
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity
语种英语
WOS记录号WOS:000338246200002
出版者BIOMED CENTRAL LTD
源URL[http://119.78.100.183/handle/2S10ELR8/277019]  
专题国家新药筛选中心
通讯作者Jin, Youxin
作者单位1.Chinese Acad Sci, Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China;
2.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China;
4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China;
5.Qidong Liver Canc Inst, Dept Etiol, Qidong 226200, Jiangsu, Peoples R China;
6.Univ Penn, Dept Radiat Oncol, Philadelphia, PA 19104 USA;
7.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Shenzhen 518055, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Botao,Yang, Dehua,Jiang, Jing,et al. Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies[J]. BMC GENOMICS,2014,15.
APA Zhao, Botao.,Yang, Dehua.,Jiang, Jing.,Li, Jinsong.,Fan, Chunsun.,...&Jin, Youxin.(2014).Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies.BMC GENOMICS,15.
MLA Zhao, Botao,et al."Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies".BMC GENOMICS 15(2014).

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。