The putative signal peptide of glucagon-like peptide-1 receptor is not required for receptor synthesis but promotes receptor expression
文献类型：期刊论文
| 作者 | Ge, Yunjun1,3; Yang, Dehua1,3 ; Dai, Antao1,3; Zhou, Caihong1,3; Zhu, Yue1,3; Wang, Ming-Wei1,2,3
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| 刊名 | BIOSCIENCE REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 34页码:717-727 |
| 关键词 | epitope tag G protein-coupled receptor glucagon-like peptide-1 receptor glycosylation signal peptide synthesis |
| ISSN号 | 0144-8463 |
| DOI | 10.1042/BSR20140120 |
| 文献子类 | Article |
| 英文摘要 | GLP-1R (glucagon-like peptide-1 receptor) mediates the 'incretin effect' and many other anti-diabetic actions of its cognate ligand, GLP-1 (glucagon-like peptide-1). It belongs to the class B family of GPCRs (G protein-coupled receptors) and possesses an N-terminal putative SP (signal peptide). It has been reported that this sequence is required for the synthesis of GLP-1R and is cleaved after receptor synthesis. In the present study, we conducted an in-depth exploration towards the role of the putative SP in GLP-1R synthesis. A mutant GLP-1R without this sequence was expressed in HEK293 cells (human embryonic kidney 293 cells) and displayed normal functionality with respect to ligand binding and activation of adenylate cyclase. Thus the putative SP does not seem to be required for receptor synthesis. Immunoblotting analysis shows that the amount of GLP-1R synthesized in HEK293 cells is low when the putative SP is absent. This indicates that the role of the sequence is to promote the expression of GLP-1R. Furthermore, epitopes tagged at the N-terminal of GLP-1R are detectable by immunofluorescence and immunoblotting in our experiments. In conclusion, the present study points to different roles of SP in GLP-1R expression which broadens our understanding of the functionality of this putative SP of GLP-1R and possibly other Class B GPCRs. |
| WOS关键词 | PROTEIN-COUPLED RECEPTORS ; ENDOPLASMIC-RETICULUM ; RECOGNITION PARTICLE ; MEMBRANE ; TRANSLOCATION ; SEQUENCES |
| 资助项目 | National Health and Family Planning Commission of China[2012ZX09304-011] ; National Health and Family Planning Commission of China[2013ZX09401003-005] ; National Health and Family Planning Commission of China[2013ZX09507001] ; National Health and Family Planning Commission of China[2013ZX09507-002] ; Shanghai Science and Technology Development Fund[13DZ2290300] ; Novo Nordisk-CAS[00000000] ; Thousand Talents Program in China[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000347799400006 |
| 出版者 | PORTLAND PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277250]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Wang, Ming-Wei |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China; 2.S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510641, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ge, Yunjun,Yang, Dehua,Dai, Antao,et al. The putative signal peptide of glucagon-like peptide-1 receptor is not required for receptor synthesis but promotes receptor expression[J]. BIOSCIENCE REPORTS,2014,34:717-727. |
| APA | Ge, Yunjun,Yang, Dehua,Dai, Antao,Zhou, Caihong,Zhu, Yue,&Wang, Ming-Wei.(2014).The putative signal peptide of glucagon-like peptide-1 receptor is not required for receptor synthesis but promotes receptor expression.BIOSCIENCE REPORTS,34,717-727. |
| MLA | Ge, Yunjun,et al."The putative signal peptide of glucagon-like peptide-1 receptor is not required for receptor synthesis but promotes receptor expression".BIOSCIENCE REPORTS 34(2014):717-727. |
入库方式： OAI收割
来源：上海药物研究所
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