Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons
文献类型：期刊论文
| 作者 | Yang, Dehua2,3,5 ; Li, Ting2,3; Xu, Minghan1; Gao, Feng1; Yang, Juan2,3; Yang, Zhi1; Le, Weidong2,3,4
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| 刊名 | NANOMEDICINE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:16页码:2445-2455 |
| ISSN号 | 1743-5889 |
| 关键词 | carbon nanotubes differentiation dopamine neurons embryonic stem cells graphene graphene oxide |
| DOI | 10.2217/NNM.13.197 |
| 文献子类 | Article |
| 英文摘要 | Aim: Nanoparticles are easier to pass through cell membranes, and they are considered to be the ideal biocompatible and mechanically stable platforms for supporting stem cell growth and differentiation. The aim of this study is to determine the effects of carbon nanotubes (CNTs), graphene oxide (GO) and graphene (GR) on the dopamine neural differentiation of mouse embryonic stem cells (ESCs). Materials & methods: GO was prepared according to a modified Hummers method. GR was synthesized by reduction of GO via l-ascorbic acid as a reductant in an aqueous solution at room temperature. CNTs were fabricated by chemical vapor deposition method. ESCs were differentiated by a stromal cell-derived inducing activity (SDIA) method after 10 days coculture with PA6 cells. The dopamine neural differentiation of the ESCs-GFP was examined by immunocytochemistry and real-time PCR. Results: We found that only GO could effectively promote dopamine neuron differentiation after induction of SDIA and further enhance dopamine neuron-related gene expression compared with cells treated with no nanoparticle control, and the other two nanoparticles (CNTs and GR). Conclusion: These findings suggest that GO is a promising nanomaterial-based technical platform to effectively enhance dopamine neural differentiation of ESCs, which can be potentially applied for cell transplantation therapy. |
| WOS关键词 | CARBON NANOTUBES ; OSTEOGENIC DIFFERENTIATION ; BIOCOMPATIBILITY ; PROLIFERATION ; NANOMATERIALS ; EXPRESSION ; DISEASE ; FILMS |
| 资助项目 | National Basic Research Program of China[2010CB945200] ; National Natural Science Foundation of China[81171201] ; Program for New Century Excellent Talents in University[NCET-12-0356] ; Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning[00000000] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | FUTURE MEDICINE LTD |
| WOS记录号 | WOS:000346177600003 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277251]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Yang, Zhi |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Key Lab Thin Film & Microfabricat, Minist Educ, Res Inst Micro Nano Sci & Technol, Shanghai 200240, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China; 4.Dalian Med Univ, Hosp 1, Ctr Translat Neurol, Dalian 116011, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201204, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yang, Dehua,Li, Ting,Xu, Minghan,et al. Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons[J]. NANOMEDICINE,2014,9(16):2445-2455. |
| APA | Yang, Dehua.,Li, Ting.,Xu, Minghan.,Gao, Feng.,Yang, Juan.,...&Le, Weidong.(2014).Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons.NANOMEDICINE,9(16),2445-2455. |
| MLA | Yang, Dehua,et al."Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons".NANOMEDICINE 9.16(2014):2445-2455. |
入库方式： OAI收割
来源：上海药物研究所
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