Natural product vindoline stimulates insulin secretion and efficiently ameliorates glucose homeostasis in diabetic murine models
文献类型：期刊论文
| 作者 | Yao, Xin-gang1; Chen, Fanglei1; Li, Ping1; Quan, Lingling2; Chen, Jing1 ; Yu, Liang1; Ding, Hong1; Li, Chenjing1; Chen, Lili1; Gao, Zhaobing1
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2013-10-28 |
| 卷号 | 150期号:1页码:285-297 |
| 关键词 | Vindoline Type 2 diabetes Glucose-stimulated insulin secretion beta-cell dysfunction |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2013.08.043 |
| 文献子类 | Article |
| 英文摘要 | Ethnopharmacological relevance: Catharanthus roseus (L). Don (Catharanthus roseus) is a traditional antidiabetic herb widely used in many countries, and the alkaloids of Catharanthus roseus are considered to possess hypoglycemic ability. Aim of the study: To systematically investigate the potential anti-diabetic effects and the underlying antidiabetic mechanisms of vindoline, one of the alkaloids in Catharanthus roseus. Materials and methods: The regulation of vindoline against the glucose-stimulated insulin secretion (GSIS) was examined in insulinoma MIN6 cells and primary pancreatic islets. Insulin concentration was detected by Elisa assay. Diabetic models of db/db mice and type 2 diabetic rats induced by high-fat diet combining with streptozotocin (STZ/HFD-induced type 2 diabetic rats) were used to evaluate the antidiabetic effect of vindoline in vivo. Daily oral treatment with vindoline (20 mg/kg) to diabetic mice/rats for 4 weeks, body weight and blood glucose were determined every week, oral glucose tolerance test (OGTT) was performed after 4 weeks. Results: Vindoline enhanced GSIS in both glucose- and dose-dependent manners (EC50=50 mu M). It was determined that vindoline acted as a Kv2.1 inhibitor able to reduce the voltage-dependent outward potassium currents finally enhancing insulin secretion. It protected beta-cells from the cytokines-induced apoptosis following its inhibitory role in Kv2.1. Moreover, vindoline (20 mg/kg) treatment significantly improved glucose homeostasis in db/db mice and STZ/HFD-induced type 2 diabetic rats, as reflected by its functions in increasing plasma insulin concentration, protecting the pancreatic beta-cells from damage, decreasing fasting blood glucose and glycated hemoglobin (HbA(1c)), improving OGTT and reducing plasma triglyceride (TG). Conclusion: Our findings suggested that vindoline might contribute to the anti-diabetic effects of Catharanthus roseus, and this natural product may find its more applications in the improvement of beta-cell dysfunction and further the potential treatment of type 2 diabetes. (C) 2013 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | PANCREATIC BETA-CELLS ; NEURONAL APOPTOSIS ; K+ CHANNELS ; CATHARANTHUS-ROSEUS ; ELECTRICAL-ACTIVITY ; KV2.1 ; MELLITUS ; HYPOGLYCEMIA ; DYSFUNCTION ; INHIBITION |
| 资助项目 | National Marine 863 Program[2011AA09070102] ; National Science and Technology Major Project[2012ZX09103101-018] ; National Science and Technology Major Project[2012ZX09301001-004] ; National Natural Science Foundation of China[30925040] ; National Natural Science Foundation of China[81273397] ; Science Foundation of Shanghai[12431900300] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000326998400032 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277415]  |
| 专题 | 药物安全性评价中心 |
| 通讯作者 | Chen, Jing |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China; 2.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Xin-gang,Chen, Fanglei,Li, Ping,et al. Natural product vindoline stimulates insulin secretion and efficiently ameliorates glucose homeostasis in diabetic murine models[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,150(1):285-297. |
| APA | Yao, Xin-gang.,Chen, Fanglei.,Li, Ping.,Quan, Lingling.,Chen, Jing.,...&Shen, Xu.(2013).Natural product vindoline stimulates insulin secretion and efficiently ameliorates glucose homeostasis in diabetic murine models.JOURNAL OF ETHNOPHARMACOLOGY,150(1),285-297. |
| MLA | Yao, Xin-gang,et al."Natural product vindoline stimulates insulin secretion and efficiently ameliorates glucose homeostasis in diabetic murine models".JOURNAL OF ETHNOPHARMACOLOGY 150.1(2013):285-297. |
入库方式： OAI收割
来源：上海药物研究所
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