中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 国家新药筛选中心
Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency

文献类型：期刊论文

作者Xu, Yongyu1,2; Wei, Xiaoyuan1,2; Wang, Min1; Zhang, Ru2; Fu, Yanbin1,2; Xing, Mingzhe1,2; Hua, Qiuhong2; Xie, Xin1,2
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2013-04-05
卷号288期号:14页码:9767-9778
ISSN号0021-9258
DOI10.1074/jbc.M112.403881
文献子类Article
英文摘要The discovery of induced pluripotent stem (iPS) cells provides not only new approaches for cell replacement therapy, but also new ways for drug screening. However, the undefined mechanism and relatively low efficiency of reprogramming have limited the application of iPS cells. In an attempt to further optimize the reprogramming condition, we unexpectedly observed that removing c-Myc from the Oct-4, Sox-2, Klf-4, and c-Myc (OSKM) combination greatly enhanced the generation of iPS cells. The iPS cells generated without c-Myc attained salient pluripotent characteristics and were capable of producing fullterm mice through tetraploid complementation. We observed that forced expression of c-Myc induced the expression of many genes involved in cell cycle control and a hyperproliferation state of the mouse embryonic fibroblasts during the early stage of reprogramming. This enhanced proliferation of mouse embryonic fibroblasts correlated negatively to the overall reprogramming efficiency. By applying small molecule inhibitors of cell proliferation at the early stage of reprogramming, we were able to improve the efficiency of iPS cell generation mediated by OSKM. Our data demonstrated that the proliferation rate of the somatic cell plays critical roles in reprogramming. Slowing down the proliferation of the original cells might be beneficial to the induction of iPS cells.
WOS关键词PLURIPOTENT STEM-CELLS ; SUBEROYLANILIDE HYDROXAMIC ACID ; DEXAMETHASONE INHIBITS PROLIFERATION ; SIGNALING PROMOTES PROLIFERATION ; HISTONE DEACETYLASE INHIBITORS ; HUMAN NEUROBLASTOMA-CELLS ; C-MYC ; SELF-RENEWAL ; CANCER-CELLS ; IN-VIVO
资助项目Chinese Academy of Sciences[XDA01040301] ; Ministry of Science and Technology of China[2009CB940900] ; Ministry of Science and Technology of China[2010CB944900] ; Ministry of Science and Technology of China[2011CB965104] ; Ministry of Science and Technology of China[2011DFB30010] ; Shanghai Commission of Science and Technology[11DZ2292200] ; Shanghai Commission of Science and Technology[12XD1402100]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000317114000018
出版者AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
源URL[http://119.78.100.183/handle/2S10ELR8/277661]  
专题国家新药筛选中心
通讯作者Zhang, Ru
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Stake Key Lab Drug Res, Shanghai 201203, Peoples R China
2.Tongji Univ, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Lab Receptor Based Biomed, Shanghai 200092, Peoples R China;
推荐引用方式
GB/T 7714		 Xu, Yongyu,Wei, Xiaoyuan,Wang, Min,et al. Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(14):9767-9778.
APA Xu, Yongyu.,Wei, Xiaoyuan.,Wang, Min.,Zhang, Ru.,Fu, Yanbin.,...&Xie, Xin.(2013).Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency.JOURNAL OF BIOLOGICAL CHEMISTRY,288(14),9767-9778.
MLA Xu, Yongyu,et al."Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency".JOURNAL OF BIOLOGICAL CHEMISTRY 288.14(2013):9767-9778.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。