Stress-mediated p38 activation promotes somatic cell reprogramming
文献类型：期刊论文
| 作者 | Xu, Xinxiu1,2; Wang, Quan1; Long, Yuan1; Zhang, Ru2; Wei, Xiaoyuan2; Xing, Mingzhe2; Gu, Haifeng1 ; Xie, Xin1,2
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| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2013-01 |
| 卷号 | 23期号:1页码:131-141 |
| 关键词 | induced pluripotent stem cells iPS hyperosmosis NaCl stress p38 reprogramming |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2012.143 |
| 文献子类 | Article |
| 英文摘要 | Environmental stress-mediated adaptation plays essential roles in the evolution of life. Cellular adaptation mechanisms usually involve the regulation of chromatin structure, transcription, mRNA stability and translation, which eventually lead to efficient changes in gene expression. Global epigenetic change is also involved in the reprogramming of somatic cells into induced pluripotent stem (iPS) cells by defined factors. Here we report that environmental stress such as hyperosmosis not only facilitates four factor-mediated reprogramming, but also enhances two or one factor-induced iPS cell generation. Hyperosmosis-induced p38 activation plays a critical role in this process. Constitutive active p38 mimics the positive effect of hyperosmosis, while dominant negative p38 and p38 inhibitor block the effect of hyperosmosis. Further study indicates stress-mediated p38 activation may promote reprogramming by reducing the global DNA methylation level and enhancing the expression of pluripotency genes. Our results demonstrate how simple environmental stress like hyperosmosis helps to alter the fate of cells via intracellular signaling and epigenetic modulation. |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; GENE-EXPRESSION ; FIBROBLASTS ; DIFFERENTIATION ; GENERATION ; INDUCTION |
| 资助项目 | Chinese Academy of Sciences[XDA01040301] ; Ministry of Science and Technology of China[2009CB940900] ; Ministry of Science and Technology of China[2010CB944900] ; Ministry of Science and Technology of China[2011CB965104] ; Ministry of Science and Technology of China[2011DFB30010] ; Shanghai Commission of Science and Technology[08DZ2291300] ; Shanghai Commission of Science and Technology[09DZ2291200] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4724487 |
| WOS记录号 | WOS:000313194700016 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277828]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Xie, Xin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Stake Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Tongji Univ, Lab Receptor Based Biomed, Shanghai Key Lab Signaling & Dis Res, Sch Life Sci & Technol, Shanghai 200092, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xu, Xinxiu,Wang, Quan,Long, Yuan,et al. Stress-mediated p38 activation promotes somatic cell reprogramming[J]. CELL RESEARCH,2013,23(1):131-141. |
| APA | Xu, Xinxiu.,Wang, Quan.,Long, Yuan.,Zhang, Ru.,Wei, Xiaoyuan.,...&Xie, Xin.(2013).Stress-mediated p38 activation promotes somatic cell reprogramming.CELL RESEARCH,23(1),131-141. |
| MLA | Xu, Xinxiu,et al."Stress-mediated p38 activation promotes somatic cell reprogramming".CELL RESEARCH 23.1(2013):131-141. |
入库方式： OAI收割
来源：上海药物研究所
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