Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice
文献类型：期刊论文
| 作者 | Wu, Mengjun1,2; Xing, Guozhen1; Qi, Xinming1 ; Feng, Chenchen1,2; Liu, Mingxia1,2; Gong, Likun1; Luan, Yang1; Ren, Jin1
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| 刊名 | MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
 |
| 出版日期 | 2012-10-09 |
| 卷号 | 748期号:1-2页码:65-69 |
| 关键词 | Arecoline gpt delta transgenic mouse Mutagenesis |
| ISSN号 | 1383-5718 |
| DOI | 10.1016/j.mrgentox.2012.07.001 |
| 文献子类 | Article |
| 英文摘要 | Chewing the areca nut is carcinogenic to humans. Arecoline, a major alkaloid in areca nut, is suspected to be a carcinogenic component. It has been shown to have genotoxic potential in various in vitro systems; but information on its in vivo genotoxicity is limited. To investigate the organ-specific mutagenic potential of arecoline, we employed gpt delta transgenic mice to analyze the mutagenicity of arecoline in the oral tissues and liver. Male gpt delta mice were given arecoline hydrobromide in drinking water at 300 and 700 mu g/mL for 6 weeks. 4-Nitroquinoline-1 (4-NQO) was used as a positive control. Two weeks after the last treatment, mutation frequencies in the oral tissues (a mixture of gingival, buccal, pharyngeal and sublingual tissue) and liver were detected and mutation spectra were analyzed. There were no statistically significant differences in the average mutation frequencies between arecoline-treated and untreated groups in both the oral tissues and liver. However, in the oral tissues, one mouse in arecoline-300 mu g/mL group and two mice in arecoline-700 mu g/mL group showed more than 2.5-fold higher mutation frequencies than the untreated group; they also exhibited unique mutation spectra compared to spontaneous mutation types. In these three mice, all mutations occurred at G:C sites, where G:C -> T:A transversions were most frequent, followed by G:C -> A:T transitions and G:C -> C:G transversions. The main type of spontaneous mutation in both the oral tissues and liver was G:C -> A:T transition. These results suggest that arecoline poses a mutagenic hazard in the oral tissues of gpt delta transgenic mice. (c) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | HUMAN GINGIVAL FIBROBLASTS ; ORAL SUBMUCOUS FIBROSIS ; BUCCAL EPITHELIAL-CELLS ; BETEL QUID CHEWERS ; ARECA NUT EXTRACT ; DNA-DAMAGE ; IN-VITRO ; OXIDATIVE-STRESS ; CARCINOGENESIS ; INGREDIENTS |
| 资助项目 | Key Projects of National Science and Technology Pillar Program[2012ZX09301001-006] ; Key Projects of National Science and Technology Pillar Program[2012zx09302003] ; Public Service Platform Project of Shanghai Science and Technology Committee[11DZ2292500] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000308515300011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277912]  |
| 专题 | 药物安全性评价中心 |
| 通讯作者 | Luan, Yang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Ctr Drug Safety & Evaluat Res, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Grad Sch, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Mengjun,Xing, Guozhen,Qi, Xinming,et al. Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2012,748(1-2):65-69. |
| APA | Wu, Mengjun.,Xing, Guozhen.,Qi, Xinming.,Feng, Chenchen.,Liu, Mingxia.,...&Ren, Jin.(2012).Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,748(1-2),65-69. |
| MLA | Wu, Mengjun,et al."Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 748.1-2(2012):65-69. |
入库方式： OAI收割
来源：上海药物研究所
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