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Microarray analysis of responsible genes in increased growth rate in the subline of HL60 (HL60RG) cells

文献类型：期刊论文

作者Luan, Yang1,3; Kogi, Mieko2,3; Rajaguru, Palanisamy3,4; Ren, Jin1; Yamaguchi, Teruhide3; Suzuki, Kazuhiro3; Suzuki, Takayoshi3
刊名MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
出版日期2012-03-01
卷号731期号:1-2页码:20-26
关键词Microarray analysis HL60 cells HL60RG cell Type II tumor necrosis factor-alpha receptor (TNFRSF1B) TNFRSF8
ISSN号0027-5107
DOI10.1016/j.mrfmmm.2011.10.005
文献子类Article
英文摘要HL60RG, a subline of human promyelocytic leukemia HL60 cells, has a increased growth rate than their parental cells. To gain information of the mechanisms involved in the increased growth rate of HL60RG, we performed a multiplex fluorescence in situ hybridization (M-FISH), standard cytogenetics analysis (G-banding) and genome scan using 10K SNP mapping array on both cell types. Characteristic genomic alterations in HL60RG cells were identified including uniparental disomy (UPD) of chromosome 1, and hemizygous deletion in 10p and 11p. However, no such defects were observed in HL60 cells. Changes in gene expression in HL60RG cells were determined using expression arrays (Affymetrix GeneChip, HU133A). Candidate genes associated with the rapid growth of HL60RG cells were identified. Two tumor necrosis factor receptors, TNFRSF1B (type II tumor necrosis factor-alpha receptor) and TNFRSF8 (also known as a tumor marker CD30), which are adjacently located on chromosome 1 showed opposing changes in gene expression in HL60RG cells over-expression of TNFRSF8 and repression of TNFRSF1B. Differences in the DNA methylation status in the transcriptional regulatory regions of both genes between HL60 and HL60RG was detected by a methylation-specific PCR assay. In conclusion, alterations in chromosome and gene expression in HL60RG may be associated with increased growth rate. (C) 2011 Elsevier B.V. All rights reserved.
WOS关键词DOUBLE MINUTE CHROMOSOMES ; MYELOID-LEUKEMIA ; C-MYC ; IMPRINTED GENES ; HL-60 CELLS ; CANCER ; DNA ; AMPLIFICATION ; POLYMORPHISM ; METHYLATION
资助项目Ministry of Health, Labor, and Welfare of Japan[00000000]
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种英语
WOS记录号WOS:000300918800003
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/278173]  
专题药物安全性评价中心
通讯作者Luan, Yang
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat, Shanghai 200031, Peoples R China;
2.Kanazawa Inst Technol, Dept Appl Biosci, Coll Biosci & Chem, Kanazawa, Ishikawa 9240838, Japan;
3.Natl Inst Hlth Sci, Div Cellular & Gene Therapy Prod, Setagaya Ku, Tokyo 1588501, Japan;
4.Anna Univ Technol, Dept Biotechnol, Tiruchchirappalli 620024, India
推荐引用方式
GB/T 7714		 Luan, Yang,Kogi, Mieko,Rajaguru, Palanisamy,et al. Microarray analysis of responsible genes in increased growth rate in the subline of HL60 (HL60RG) cells[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2012,731(1-2):20-26.
APA Luan, Yang.,Kogi, Mieko.,Rajaguru, Palanisamy.,Ren, Jin.,Yamaguchi, Teruhide.,...&Suzuki, Takayoshi.(2012).Microarray analysis of responsible genes in increased growth rate in the subline of HL60 (HL60RG) cells.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,731(1-2),20-26.
MLA Luan, Yang,et al."Microarray analysis of responsible genes in increased growth rate in the subline of HL60 (HL60RG) cells".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 731.1-2(2012):20-26.

入库方式： OAI收割

来源：上海药物研究所

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