Quantitative Proteomic Analysis of Membrane Proteins Involved in Astroglial Differentiation of Neural Stem Cells by SILAC Labeling Coupled with LC-MS/MS
文献类型：期刊论文
| 作者 | Cao, Rui1; Chen, Ke1; Song, Qin1; Zang, Yi2 ; Li, Jia2; Wang, Xianchun1; Chen, Ping1; Liang, Songping1
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
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| 出版日期 | 2012-02 |
| 卷号 | 11期号:2页码:829-838 |
| 关键词 | neural stem cell astroglial differentiation membrane protein SILAC tandem mass spectrometry energy metabolic pathway |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr200677z |
| 文献子类 | Article |
| 英文摘要 | Membrane proteins play a critical role in the process of neural stem cell self-renewal and differentiation. Here, we apply the SILAC (stable isotope labeling by amino acids in cell culture) approach to quantitatively compare the membrane proteome of the self-renewing and the astroglial differentiating cells. High-resolution analysis on a linear ion trap-Orbitrap instrument (LTQ-Orbitrap) at sub-ppm mass accuracy resulted in confident identification and quantitation of more than 700 distinct membrane proteins during the astroglial differentiation. Of the 735 quantified proteins, seven cell surface proteins display significantly higher expression levels in the undifferentiated state membrane compared to astroglial differentiating membrane. One cell surface protein transferrin receptor protein 1 may serve as a new candidate for NSCs surface markers. Functional clustering of differentially expressed proteins by Ingenuity Pathway Analysis revealed that most of overexpressed membrane proteins in the astroglial differentiation neural stem cells are involved in cellular growth, nervous system development, and energy metabolic pathway. Taken together, this study increases our understanding of the underlying mechanisms that modulate complex biological processes of neural stem cell proliferation and differentiation. |
| WOS关键词 | OSTEOBLAST DIFFERENTIATION ; CULTURE SILAC ; AMINO-ACIDS ; MOUSE-BRAIN ; ANNEXIN-V ; IN-VITRO ; FIBRONECTIN ; RAT ; PATHWAY ; KINASE |
| 资助项目 | National Natural Science Foundation of China[31000375] ; National Natural Science Foundation of China[81070353] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000300458300027 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278190]  |
| 专题 | 国家新药筛选中心 药物安全性评价中心 |
| 通讯作者 | Cao, Rui |
| 作者单位 | 1.Hunan Normal Univ, Coll Life Sci, Educ Comm, Key Lab Prot Chem & Dev Biol, Changsha 410081, Hunan, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Rui,Chen, Ke,Song, Qin,et al. Quantitative Proteomic Analysis of Membrane Proteins Involved in Astroglial Differentiation of Neural Stem Cells by SILAC Labeling Coupled with LC-MS/MS[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(2):829-838. |
| APA | Cao, Rui.,Chen, Ke.,Song, Qin.,Zang, Yi.,Li, Jia.,...&Liang, Songping.(2012).Quantitative Proteomic Analysis of Membrane Proteins Involved in Astroglial Differentiation of Neural Stem Cells by SILAC Labeling Coupled with LC-MS/MS.JOURNAL OF PROTEOME RESEARCH,11(2),829-838. |
| MLA | Cao, Rui,et al."Quantitative Proteomic Analysis of Membrane Proteins Involved in Astroglial Differentiation of Neural Stem Cells by SILAC Labeling Coupled with LC-MS/MS".JOURNAL OF PROTEOME RESEARCH 11.2(2012):829-838. |
入库方式： OAI收割
来源：上海药物研究所
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