Differential distributions and trafficking properties of dopamine D1 and D5 receptors in nerve cells
文献类型：期刊论文
| 作者 | He, You; Yu, Lei-Ping; Jin, Guo-Zhang
|
| 刊名 | NEUROSCIENCE BULLETIN
 |
| 出版日期 | 2009-04 |
| 卷号 | 25期号:2页码:43-53 |
| 关键词 | dopamine D1 receptor dopamine D5 receptor trafficking internalization green fluorescent protein SKF38393 acetylcholine |
| ISSN号 | 1673-7067 |
| DOI | 10.1007/s12264-009-0107-0 |
| 文献子类 | Article |
| 英文摘要 | Objective To explore the possible differential trafficking properties of the dopamine D1-like receptor subtypes, D1 receptor and D5 receptor. Methods To visualize distributions of dopamine D1-like receptor subtypes at subcellular level, the yellow and cyan variants of green fluorescent protein (GFP) were used to tag D1 and D5 receptors. After transfection with the tagged dopamine receptors, the neuroblastoma cells NG108-15 were treated with D1 agonist SKF38393 or acetylcholine (ACh). Then we observed the subcellular distributions of the tagged receptors under the confocal microscopy and tried to determine trafficking properties by comparing their distribution patterns before and after the drug treatment. Results In resting conditions, D1 receptors located in the plasma membrane of NG108-15 cells, while D5 receptors located in both plasma membrane and cytosol. With the pre-treatment of SKF38393, the subcellular distribution of D1 receptors was changed. The yellow particle-like fluorescence of tagged D1 receptors appeared in the cytosol, indicating that D1 receptors were internalized into cytosol from the cell surface. Same situation also occurred in ACh pre-treatment. In contrast, the subcellular distribution of D5 receptors was not changed after SKF38393 or ACh treatment, indicating that D5R was not translocated to cell surface. Interestingly, when D1 and D5 receptors were co-expressed in the same cell, both kept their distinct subcellular distribution patterns and the trafficking properties. Conclusion Our present study reveals that in NG108-15 nerve cells, dopamine D1 and D5 receptors exhibit differential subcellular distribution patterns, and only D1 receptor has a marked trafficking response to the drug stimulation. We further discuss the potential role of the differential trafficking properties of D1-like receptors in complex modulation of DA signaling. |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000208089000001 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279285]  |
| 专题 | 院士及顾问专家 |
| 通讯作者 | Jin, Guo-Zhang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, You,Yu, Lei-Ping,Jin, Guo-Zhang. Differential distributions and trafficking properties of dopamine D1 and D5 receptors in nerve cells[J]. NEUROSCIENCE BULLETIN,2009,25(2):43-53. |
| APA | He, You,Yu, Lei-Ping,&Jin, Guo-Zhang.(2009).Differential distributions and trafficking properties of dopamine D1 and D5 receptors in nerve cells.NEUROSCIENCE BULLETIN,25(2),43-53. |
| MLA | He, You,et al."Differential distributions and trafficking properties of dopamine D1 and D5 receptors in nerve cells".NEUROSCIENCE BULLETIN 25.2(2009):43-53. |
入库方式： OAI收割
来源：上海药物研究所
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