Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies
文献类型：期刊论文
| 作者 | Zhong, Wenjing1,2; Li, Guanghui1; Yu, Xiaolu1; Zhu, Min1; Gong, Likun1 ; Wan, Yakun1
|
| 刊名 | MICROBIOLOGYOPEN
 |
| 出版日期 | 2018-08 |
| 卷号 | 7期号:4 |
| 关键词 | biotin-streptavidin system Cry1B nanobody phage display sandwich ELISA |
| ISSN号 | 2045-8827 |
| DOI | 10.1002/mbo3.581 |
| 文献子类 | Article |
| 英文摘要 | Bt Cry1B toxin, a residue in insect-resistant transgenic plants, has been identified to be harmful to human health. Therefore, it is urgent to detect the Cry1B toxin level in each kind of transgenic plant. Nbs, with prominently unique physiochemical properties, are becoming more and more promising tools in the detection of target antigens. In this study, an immune phage display library that was of high quality was successfully constructed for the screening of Cry1B-specific Nbs with excellent specificity, affinity, and thermostable. Subsequently, a novel sandwich ELISA for Cry1B detection was established, which was based on the biotin-streptavidin system using these afore-mentioned Nbs. This established detection system presented a linear working range from 5 to 1000 ng ml(-1) and a low detection limit of 3.46 ng ml(-1). The recoveries from spiked samples were in the range of 82.51%-113.56% with a relative standard deviation (RSD) lower than 5.00%. Taken together, the proposed sandwich ELISA would be a potential method for the detection of Cry1B toxin in transgenic Bt plants specifically and sensitively. |
| WOS关键词 | PHAGE-DISPLAYED LIBRARY ; DIRECTED EVOLUTION ; NANOBODY ; CONSTRUCTION ; IMMUNOASSAY ; CHEMISTRY ; PROTEINS ; AFFINITY |
| 资助项目 | Chinese Academy of Sciences[XDA12020332] ; Chinese Academy of Sciences[CASIMM0120164005] ; National Natural Science Foundation of China[31471216] ; National Natural Science Foundation of China[81673344] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000440928500004 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279637]  |
| 专题 | 药物安全性评价中心 |
| 通讯作者 | Zhu, Min; Gong, Likun; Wan, Yakun |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Wenjing,Li, Guanghui,Yu, Xiaolu,et al. Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies[J]. MICROBIOLOGYOPEN,2018,7(4). |
| APA | Zhong, Wenjing,Li, Guanghui,Yu, Xiaolu,Zhu, Min,Gong, Likun,&Wan, Yakun.(2018).Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies.MICROBIOLOGYOPEN,7(4). |
| MLA | Zhong, Wenjing,et al."Sensitive detection of Bacillus thuringiensis Cry1B toxin based on camel single-domain antibodies".MICROBIOLOGYOPEN 7.4(2018). |
入库方式： OAI收割
来源：上海药物研究所
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