Lower genomic stability of induced pluripotent stem cells reflects increased non-homologous end joining
文献类型：期刊论文
| 作者 | Zhang, Minjie1,5; Wang, Liu4; An, Ke1,5; Cai, Jun1; Li, Guochao1,5; Yang, Caiyun1; Liu, Huixian1; Du, Fengxia1; Han, Xiao1,5; Zhang, Zilong1,5 |
| 刊名 | CANCER COMMUNICATIONS
 |
| 出版日期 | 2018-07-25 |
| 卷号 | 38 |
| 关键词 | Genomic stability DNA damage repair iPSCs ESCs |
| ISSN号 | 2523-3548 |
| DOI | 10.1186/s40880-018-0313-0 |
| 文献子类 | Article |
| 英文摘要 | Background: Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) share many common features, including similar morphology, gene expression and in vitro differentiation profiles. However, genomic stability is much lower in iPSCs than in ESCs. In the current study, we examined whether changes in DNA damage repair in iPSCs are responsible for their greater tendency towards mutagenesis. Methods: Mouse iPSCs, ESCs and embryonic fibroblasts were exposed to ionizing radiation (4 Gy) to introduce double-strand DNA breaks. At 4 h later, fidelity of DNA damage repair was assessed using whole-genome re-sequencing. We also analyzed genomic stability in mice derived from iPSCs versus ESCs. Results: In comparison to ESCs and embryonic fibroblasts, iPSCs had lower DNA damage repair capacity, more somatic mutations and short indels after irradiation. iPSCs showed greater non-homologous end joining DNA repair and less homologous recombination DNA repair. Mice derived from iPSCs had lower DNA damage repair capacity than ESC-derived mice as well as C57 control mice. Conclusions: The relatively low genomic stability of iPSCs and their high rate of tumorigenesis in vivo appear to be due, at least in part, to low fidelity of DNA damage repair. |
| WOS关键词 | SMALL-MOLECULE COMPOUNDS ; DEFECTIVE-DNA REPAIR ; DOUBLE-STRAND BREAKS ; MOUSE SOMATIC-CELLS ; HOMOLOGOUS RECOMBINATION ; IPS CELLS ; HUMAN FIBROBLASTS ; MAMMALIAN-CELLS ; DEFINED FACTORS ; READ ALIGNMENT |
| 资助项目 | Chinese Academy of Sciences[KJZD-EW-L14] ; Chinese Academy of Sciences[XDA01040407] ; National Natural Science Foundation of China[31471395] ; National Natural Science Foundation of China[91019024] ; National Natural Science Foundation of China[31540033] ; National Natural Science Foundation of China[31100558] ; National Basic Research Program of China (973 Program)[2012CB518302] ; National Basic Research Program of China (973 Program)[2013CB911001] ; 100 Talents Project[00000000] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000439889000001 |
| 出版者 | BMC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279652]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Sun, Yingli |
| 作者单位 | 1.Chinese Acad Sci, China Gastrointestinal Canc Res Ctr, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Key Lab Regenerat Biol, Guangdong Prov Key Lab Stem Cell & Regenerat, South China Inst Stem Cell Biol & Regenerat Med,G, Guangzhou 510530, Guangdong, Peoples R China; 4.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China; 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Minjie,Wang, Liu,An, Ke,et al. Lower genomic stability of induced pluripotent stem cells reflects increased non-homologous end joining[J]. CANCER COMMUNICATIONS,2018,38. |
| APA | Zhang, Minjie.,Wang, Liu.,An, Ke.,Cai, Jun.,Li, Guochao.,...&Sun, Yingli.(2018).Lower genomic stability of induced pluripotent stem cells reflects increased non-homologous end joining.CANCER COMMUNICATIONS,38. |
| MLA | Zhang, Minjie,et al."Lower genomic stability of induced pluripotent stem cells reflects increased non-homologous end joining".CANCER COMMUNICATIONS 38(2018). |
入库方式： OAI收割
来源：上海药物研究所
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