Biomass-derived N, S, P-triple-doped porous carbon materials are widely considered as one of the most promising electrode materials for electrochemical detection of pharmaceutical compounds and food analysis. In this regard, we have prepared Elaeagnus gum-derived porous carbon electrodes for use in the simple electrochemical detection of metronidazole. Heteroatom (nitrogen, sulfur, phosphorus) triple-doped functionalized porous carbon synthesized based on the use of simple pyrolysis and high temperature carbonization methods without any hard templates, and was then characterized by several methods, such as scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy. The electro-analytical performance of the proposed NSP-PC modified electrode was assessed by electro-analytical technique, such as cyclic voltammetry, linear sweep voltammetry. The results indicate that under optimized conditions, this novel constructed sensor shows excellent electrochemical properties toward metronidazole determination. The metronidazole reduction process has two linear dynamic ranges from 0.1 to 45 mu M and 50 to 350 mu M and a low detection limit of 0.013 mu M. The constructed sensor was successfully applied for detect of metronidazole in pharmaceutical, milk samples, where it also yielded acceptable results.