Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax
文献类型：期刊论文
| 作者 | Yin, Ying1; Yu, Weili2; Li, Yujie1; Liu, Kun1; Zai, Xiaodong1; Zhang, Jun1; Fu, Ling1; Hu, Tao2; Xu, Junjie1; Chen, Wei1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2019-09-15 |
| 卷号 | 137页码:790-800 |
| 关键词 | Antigen delivery system Anthrax Self-conjugation Polyethylene glycol |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2019.06.233 |
| 英文摘要 | Anthrax is an acute and highly lethal disease caused by Bacillus anthracis. Protective antigen (PA) is the primary candidate antigen for the anthrax vaccines. However, PA suffers from poor immunogenicity with short-term anti PA antibody response. High effectiveness, durable immunity, and minimal risk are required for development of an effective anthrax vaccine. In the present study, PA was self-conjugated by 8-arm polyethylene glycol (PEG) and further by thioester chemistry. As a result, 3-5 PA molecules were covalently conjugated and functioned as an antigen delivery system. The conjugate (PA-PEG) could maintain the structural properties of PA and increase the thermal stability of PA. PA-PEG could elicit a robust anti-PA IgG and neutralization antibody response in the magnitude and quality. The antibodies could be largely maintained for 180 days after three immunizations of PA-PEG. PA-PEG effectively stimulated the maturation of dendritic cell and rapidly induced the germinal center (GC) reaction. The percentages of the GC B-cells and T follicular helper (Tfh) cells were thus significantly augmented. The inflammatory response elicited by PA-PEG was comparable to those by PBS and PA. Therefore, PA-PEG is expected as an effective anthrax vaccine candidate with durable immunoprotection against anthrax. (C) 2019 Elsevier B.V. All rights reserved. |
| WOS关键词 | INHALATIONAL ANTHRAX ; DELTA INULIN ; VACCINE ; IMMUNOGENICITY ; PEGYLATION ; IMMUNITY ; CELLS ; ADVAX(TM) ; IMPROVES ; SYSTEM |
| 资助项目 | National Science and Technology Major Project of China[2016ZX10004001] ; National Natural Science Foundation of China[81703445] ; National Natural Science Foundation of China[81700181] ; National Natural Science Foundation of China[31300760] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000484644100086 |
| 出版者 | ELSEVIER |
| 资助机构 | National Science and Technology Major Project of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/30751]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Hu, Tao; Xu, Junjie; Chen, Wei |
| 作者单位 | 1.Beijing Inst Biotechnol, Lab Vaccine & Antibody Engn, Beijing 100071, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Ying,Yu, Weili,Li, Yujie,et al. Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,137:790-800. |
| APA | Yin, Ying.,Yu, Weili.,Li, Yujie.,Liu, Kun.,Zai, Xiaodong.,...&Chen, Wei.(2019).Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,137,790-800. |
| MLA | Yin, Ying,et al."Self-conjugated protective antigen elicits strong and durable protective antibody response against anthrax".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 137(2019):790-800. |
入库方式： OAI收割
来源：过程工程研究所
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