Ag85b/ESAT6-CFP10 adjuvanted with aluminum/poly-IC effectively protects guinea pigs from latent mycobacterium tuberculosis infection
文献类型：期刊论文
| 作者 | Wang, Chunhua1,3; Lu, Jinbiao1; Du, Weixin1; Wang, Guozhi1; Li, Xuguang2; Shen, Xiaobin1; Su, Cheng1; Yang, Lei1; Chen, Baowen1; Wang, Junzhi1 |
| 刊名 | VACCINE
 |
| 出版日期 | 2019-07-26 |
| 卷号 | 37期号:32页码:4477-4484 |
| 关键词 | Mycobacterium tuberculosis Subunit vaccine Adjuvant Immunogenicity Protective efficacy Latent tuberculosis infection |
| ISSN号 | 0264-410X |
| DOI | 10.1016/j.vaccine.2019.06.078 |
| 英文摘要 | The high global burden of tuberculosis (TB) underscores the urgent need for an effective TB vaccine since the only licensed Bacillus Calmette-Guerin (BCG) vaccine is ineffective in preventing adult pulmonary TB and affords no protection against latent TB infection (LTBI). Herein we investigated the potential of Mycobacterium tuberculosis (Mtb) antigen proteins AEC comprised of Ag85b and ESAT6-CFP10 proteins in conjunction with aluminum (Al) and polyriboinosinic-polyribocytidylic acid (poly-IC) as a novel subunit vaccine against TB. The immunogenicity and protection induced by the adjuvanted vaccine were evaluated in two animal models. Mice vaccinated with AEC/Al/poly-IC exhibited significant antigen-specific humoral immune responses and cell-mediated immunity as determined by immunoassay and multicolor flow cytometric assay, and the protective effect of the vaccine was demonstrated in a guinea pig model of latent Mtb infection. Compared to the control group, the mean pathological scores and bacterial loads in lungs and spleens of AEC/Al/poly-IC-immunized guinea pigs were significantly reduced. These data indicate that the AEC/Al/poly-IC is highly immunogenic in mice and can effectively protect guinea pigs against latent Mtb infection; it may represent a promising candidate vaccine for the control of latent TB. (C) 2019 Elsevier Ltd. All rights reserved. |
| WOS关键词 | CALMETTE-GUERIN VACCINATION ; CD8(+) T-CELLS ; BCG VACCINE ; MICE ; PREVENTION ; RESPONSES ; EFFICACY ; METAANALYSIS ; EPIDEMIC ; ANTIGENS |
| 资助项目 | China Mega-Project for Infectious Disease[2012ZX10004701003] |
| WOS研究方向 | Immunology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000483415000011 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | China Mega-Project for Infectious Disease |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/30822]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wang, Junzhi; Xu, Miao |
| 作者单位 | 1.Natl Inst Food & Drug Control, Div TB Vaccines, Beijing 102629, Peoples R China 2.Hlth Canada, Ctr Biol Evaluat, Biol & Genet Therapies Directorate, Ottawa, ON, Canada 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chunhua,Lu, Jinbiao,Du, Weixin,et al. Ag85b/ESAT6-CFP10 adjuvanted with aluminum/poly-IC effectively protects guinea pigs from latent mycobacterium tuberculosis infection[J]. VACCINE,2019,37(32):4477-4484. |
| APA | Wang, Chunhua.,Lu, Jinbiao.,Du, Weixin.,Wang, Guozhi.,Li, Xuguang.,...&Xu, Miao.(2019).Ag85b/ESAT6-CFP10 adjuvanted with aluminum/poly-IC effectively protects guinea pigs from latent mycobacterium tuberculosis infection.VACCINE,37(32),4477-4484. |
| MLA | Wang, Chunhua,et al."Ag85b/ESAT6-CFP10 adjuvanted with aluminum/poly-IC effectively protects guinea pigs from latent mycobacterium tuberculosis infection".VACCINE 37.32(2019):4477-4484. |
入库方式： OAI收割
来源：过程工程研究所
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