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In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche

文献类型：期刊论文

作者Song, Kedong4; Kong, Qian4; Li, Liying4; Wang, Yiwei5; Parungao, Roxanne5; Zheng, Shuangshuang2,6; Nie, Yi2,6; Jiao, Zeren1; Wang, Hongfei3; Liu, Tianqing4
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
出版日期2019-10-01
卷号189期号:2页码:471-484
ISSN号0273-2289
关键词Osteoblasts Biomimetic niche Hematopoietic stem cell expansion Dynamic environment Cell function maintain
DOI10.1007/s12010-019-03015-z
英文摘要A novel HAp-CS/Gel biomimetic osteoblastic niche was fabricated by freeze-drying, and its mechanical strength and biocompatibility were characterized. HAp-CS/Gel scaffolds in various ratios of 100:5, 100:10, and 100:20 (CS/Gel to HAp) were prepared by freeze-drying prior to chemical cross-linking followed by infrared spectrum analysis, EDS, FITR, SEM, fluorescence microscopy, MTT, and ALP experiments. Results from the infrared spectrum analysis showed that HAp doping remained the surface morphology and the architecture of scaffold with interconnected pores in the size range of 135 to 150 mu m. The HAp doping ratio of 100:20 was found to be optimal based on its high porosity of 90%, better water uptake folds of 19.1. In addition, EDS and FITR analyses demonstrated that HAps were uniformly distributed on the surface of a scaffold with aggregates and particles, which has sufficient roughness for cell attachment and proliferation of osteoblasts. Under SEM and fluorescent microscopy, osteoblasts seeded onto the scaffold showed evenly distributed viable cells, which is believed to form a biomimetic niche. In the present study, we further demonstrate that osteoblasts can maintain their function and grow well on the scaffold through MTT and ALP tests. Thus, the scaffold has favorable physical properties and biocompatibility to support the proliferation and differentiation of osteoblasts and further to support the constructs of biomimetic osteoblastic niche.
WOS关键词HEMATOPOIETIC STEM-CELL ; SCAFFOLDS ; CHITOSAN ; GELATIN ; WOLLASTONITE ; PROTEINS
资助项目National Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
出版者SPRINGER
WOS记录号WOS:000487133300011
资助机构National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning
源URL[http://ir.ipe.ac.cn/handle/122111/31024]  
专题中国科学院过程工程研究所
通讯作者Song, Kedong; Nie, Yi; Wang, Hongfei; Liu, Tianqing
作者单位1.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Dalian Med Univ, Dept Orthoped, Affiliated Hosp 2, Dalian 116024, Peoples R China
4.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
5.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia
6.Zhengzhou Inst Emerging Technol Ind, Zhengzhou 450000, Henan, Peoples R China
推荐引用方式
GB/T 7714		 Song, Kedong,Kong, Qian,Li, Liying,et al. In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,189(2):471-484.
APA Song, Kedong.,Kong, Qian.,Li, Liying.,Wang, Yiwei.,Parungao, Roxanne.,...&Liu, Tianqing.(2019).In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,189(2),471-484.
MLA Song, Kedong,et al."In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 189.2(2019):471-484.

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来源：过程工程研究所

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