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Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method

文献类型：期刊论文

作者Zhao, Xi1; Huang, Lan1,2; Wu, Jie1; Huang, Yong-Dong1; Zhao, Lan1; Wu, Nan1; Zhou, Wei-Qing1; Hao, Dong-Xia1; Ma, Guang-Hui1,3; Su, Zhi-Guo1
刊名COLLOIDS AND SURFACES B-BIOINTERFACES
出版日期2019-10-01
卷号182页码:8
关键词Agarose microspheres Pre-cross-linking Surfactant micelles swelling method Macropores High-speed chromatography
ISSN号0927-7765
DOI10.1016/j.colsurfb.2019.110377
英文摘要A novel combined method of pre-cross-linking and surfactant micelles swelling was proposed in this study to fabricate highly cross-linked and macroporous agarose (HMA) microspheres. Agarose was chemically modified by allylglycidyl ether (AGE) as heterobifunctional cross-linker via its active glycidyl moieties before gel formation and pre-cross-linking was achieved. By this means, the effective concentration of cross-linker presented in agarose gel increased significantly, and thus cross-linking with a high-efficiency was achieved. Further to enhance the intraparticle mass transfer of agarose microspheres, the surfactant micelles swelling method was utilized to create interconnected macropores. Under the optimal condition, HMA microspheres with homogeneous reticular structure and pore size of hundreds nanometers were successfully prepared. They exhibited a low backpressure with a flow velocity as high as 1987 cm/h, which was much higher than that of commercial Sepharose 4 F F. HMA microspheres were then derivatized with carboxymethyl (CM) groups and applied in ion-exchange chromatography. As expected, CM-HMA column separated model proteins effectively even at a flow velocity three times higher than that of commercial CM-4 F F. Visualization of dynamic protein adsorption by confocal laser scanning microscope (CLSM) revealed that the intraparticle mass transfer of CM-HMA micro spheres was intensified due to its macroporous structure. All of the results indicated the newly developed agarose microspheres were a promising medium for high-speed chromatography.
WOS关键词PORE-SIZE DISTRIBUTIONS ; GIGAPOROUS POLYSTYRENE MICROSPHERES ; GEL-BOUND ENZYMES ; SUPERPOROUS AGAROSE ; PERFUSION CHROMATOGRAPHY ; HIGH-CAPACITY ; SEPARATION ; CELLULOSE ; BEADS ; ELECTROPHORESIS
资助项目National Key Research and Development Program of China[2018YFC0311101] ; National Natural Science Foundation of China (NSFC) project[21821005] ; National Natural Science Foundation of China (NSFC) project[21476241] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-218] ; Beijing Municipal Natural Science Foundation of China[2172054]
WOS研究方向Biophysics ; Chemistry ; Materials Science
语种英语
WOS记录号WOS:000489190200044
出版者ELSEVIER
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China (NSFC) project ; Key Research Program of the Chinese Academy of Sciences ; Beijing Municipal Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/31195]  
专题中国科学院过程工程研究所
通讯作者Wu, Jie; Ma, Guang-Hui
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Xi,Huang, Lan,Wu, Jie,et al. Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,182:8.
APA Zhao, Xi.,Huang, Lan.,Wu, Jie.,Huang, Yong-Dong.,Zhao, Lan.,...&Su, Zhi-Guo.(2019).Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method.COLLOIDS AND SURFACES B-BIOINTERFACES,182,8.
MLA Zhao, Xi,et al."Fabrication of rigid and macroporous agarose microspheres by pre-cross-linking and surfactant micelles swelling method".COLLOIDS AND SURFACES B-BIOINTERFACES 182(2019):8.

入库方式： OAI收割

来源：过程工程研究所

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