gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems
文献类型：期刊论文
| 作者 | Liu Zhini1; Yang Liangrong2; Dong Tingting2; Li Wensong1; Sun Xitong2; Zhu Menghao2; Duan Zhengkang1; Liu Qingfen2; Liu Huizhou2 |
| 刊名 | particuology
 |
| 出版日期 | 2016 |
| 卷号 | 24期号:FEB页码:170 |
| ISSN号 | 1674-2001 |
| 英文摘要 | In this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry. (C) 2015 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved. |
| 资助项目 | [National Key Natural Science Foundation of China] ; [National Natural Science Foundation of China] |
| 语种 | 英语 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/33151]  |
| 专题 | 中国科学院过程工程研究所 |
| 作者单位 | 1.湘潭大学 2.中国科学院过程工程研究所 |
| 推荐引用方式 GB/T 7714 | Liu Zhini,Yang Liangrong,Dong Tingting,et al. gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems[J]. particuology,2016,24(FEB):170. |
| APA | Liu Zhini.,Yang Liangrong.,Dong Tingting.,Li Wensong.,Sun Xitong.,...&Liu Huizhou.(2016).gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems.particuology,24(FEB),170. |
| MLA | Liu Zhini,et al."gasassistedmagneticseparationforthepurificationofproteinsinbatchsystems".particuology 24.FEB(2016):170. |
入库方式： OAI收割
来源：过程工程研究所
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