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Chronic exposure to graphene oxide (GO) induced inflammation and differentially disturbed the intestinal microbiota in zebrafish

文献类型：期刊论文

作者Jia, Pan-Pan3,4; Sun, Tai4; Junaid, Muhammad2,4; Xiong, Yang-Hui4; Wang, Yang-Qing4; Liu, Li3,4; Pu, Shi-Ya3,4; Pei, De-Sheng1,3,4
刊名ENVIRONMENTAL SCIENCE-NANO
出版日期2019-08-01
卷号6期号:8页码:2452-2469
ISSN号2051-8153
DOI10.1039/c9en00364a
通讯作者Pei, De-Sheng(deshengpei@gmail.com)
英文摘要Graphene oxide (GO) has been extensively used in various critical fields such as biomedicine, while its antibacterial properties and potential toxicities raised serious concerns in recent years. The impacts of GO on the intestinal microbiota and associated immune responses in the host are still ambiguous. In this study, we investigated the effects of GO chronic exposure (25 days) on the composition of the intestinal microbiota and immune response in female & male zebrafish at different concentrations (0.05, 0.5, and 5 mg L-1). Results demonstrated that the diversity and richness of the bacterial community at the phylum and genus levels significantly differed in the presence of GO between female and male zebrafish. The in vitro assay verified the effect of acute toxicity of GO on the survival ability of bacterial strains isolated from zebrafish intestines, albeit no changes in the bacterial metabolic functions were observed. Further, exposure to GO significantly altered the length and width of villi in the anterior intestines, caused abnormalities of the ultra-micro structure, and increased the number of inflammation-related cells detected by transmission electron microscopy (TEM). Moreover, the elevated expression levels of crucial pro-inflammatory genes, such as tnf-alpha, ifn-gamma, nf-kappa b, tlr-5, il-1 beta, and il-8, were significantly induced, while the expression levels of anti-inflammatory genes, such as il-4 and il-13, were inhibited, especially in male zebrafish. Taken all together, chronic exposure to GO disturbed the diversity and richness of intestinal microbes, increased the pathogenic bacterial community in zebrafish, induced damage to the gut tissues, and activated the inflammation response. These findings provided new insights into GO effects on the inflammation and intestinal microbiota in the host, which is closely linked with metabolisms and physiological activities in biological systems.
资助项目CAS Team Project of the Belt and Road ; Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Youth Innovation Program of Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences in 2018[Y83A160S10] ; Program of China-Sri Lanka Joint Research and Demonstration Center for Water Technology and China-Sri Lanka Joint Center for Education and Research by Chinese Academy of Sciences, China
WOS研究方向Chemistry ; Environmental Sciences & Ecology ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000479313800006
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.138/handle/2HOD01W0/9545]  
专题环境与健康研究中心
通讯作者Pei, De-Sheng
作者单位1.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China
2.Peking Univ, Sch Environm & Energy, Key Lab Heavy Met Pollut Control & Reutilizat, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100019, Peoples R China
4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm, Chongqing 400714, Peoples R China
推荐引用方式
GB/T 7714		 Jia, Pan-Pan,Sun, Tai,Junaid, Muhammad,et al. Chronic exposure to graphene oxide (GO) induced inflammation and differentially disturbed the intestinal microbiota in zebrafish[J]. ENVIRONMENTAL SCIENCE-NANO,2019,6(8):2452-2469.
APA Jia, Pan-Pan.,Sun, Tai.,Junaid, Muhammad.,Xiong, Yang-Hui.,Wang, Yang-Qing.,...&Pei, De-Sheng.(2019).Chronic exposure to graphene oxide (GO) induced inflammation and differentially disturbed the intestinal microbiota in zebrafish.ENVIRONMENTAL SCIENCE-NANO,6(8),2452-2469.
MLA Jia, Pan-Pan,et al."Chronic exposure to graphene oxide (GO) induced inflammation and differentially disturbed the intestinal microbiota in zebrafish".ENVIRONMENTAL SCIENCE-NANO 6.8(2019):2452-2469.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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