Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation
文献类型：期刊论文
| 作者 | Wang JW(王家文)1,2,3; Wu Y(武亿)1,2,3; Zhang X(张潇)1,2,3; Zhang F(张帆)1,2,3; Lv DY(吕东媛)1,2,3; Bing SG2,3; Gao YX(高宇欣)2,3; Long M(龙勉)1,2,3; Zhang F(张帆) ; Long M(龙勉)
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| 刊名 | STEM CELL RESEARCH & THERAPY
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| 出版日期 | 2019-11-27 |
| 卷号 | 10期号:1页码:16 |
| 关键词 | Embryonic stem cell Fluid shear mechanomics Nuclear spreading Histone acetylation Chromatin decondensation |
| DOI | 10.1186/s13287-019-1454-z |
| 通讯作者 | Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Background Distinct mechanical stimuli are known to manipulate the behaviors of embryonic stem cells (ESCs). Fundamental rationale of how ESCs respond to mechanical forces and the potential biological effects remain elusive. Here we conducted the mechanobiological study for hESCs upon mechanomics analysis to unravel typical mechanosensitive processes on hESC-specific fluid shear. Methods hESC line H1 was subjected to systematically varied shear flow, and mechanosensitive proteins were obtained by mass spectrometry (MS) analysis. Then, function enrichment analysis was performed to identify the enriched gene sets. Under a steady shear flow of 1.1 Pa for 24 h, protein expressions were further detected using western blotting (WB), quantitative real-time PCR (qPCR), and immunofluorescence (IF) staining. Meanwhile, the cells were treated with 200 nM trichostatin (TSA) for 1 h as positive control to test chromatin decondensation. Actin, DNA, and RNA were then visualized with TRITC-labeled phalloidin, Hoechst 33342, and SYTO (R) RNASelect (TM) green fluorescent cell stain (Life Technologies), respectively. In addition, cell stiffness was determined with atomic force microscopy (AFM) and annexin V-PE was used to determine the apoptosis with a flow cytometer (FCM). Results Typical mechanosensitive proteins were unraveled upon mechanomics analysis under fluid shear related to hESCs in vivo. Functional analyses revealed significant alterations in histone acetylation, nuclear size, and cytoskeleton for hESC under shear flow. Shear flow was able to induce H2B acetylation and nuclear spreading by CFL2/F-actin cytoskeletal reorganization. The resulting chromatin decondensation and a larger nucleus readily accommodate signaling molecules and transcription factors. Conclusions Shear flow regulated chromatin dynamics in hESCs via cytoskeleton and nucleus alterations and consolidated their primed state. |
| 分类号 | 一类 |
| WOS关键词 | EMBRYONIC STEM-CELLS ; EPITHELIAL-MESENCHYMAL TRANSITION ; SHEAR-STRESS ; BIOPHYSICAL REGULATION ; ENDOTHELIAL-CELLS ; HISTONE ACETYLATION ; MECHANICAL CONTROL ; ACTIN DYNAMICS ; SELF-RENEWAL ; FORCES |
| 资助项目 | National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31470907] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] |
| WOS研究方向 | Cell Biology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000499837400001 |
| 资助机构 | National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy |
| 其他责任者 | Long, Mian |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/81206]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 3.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang JW,Wu Y,Zhang X,et al. Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation[J]. STEM CELL RESEARCH & THERAPY,2019,10(1):16. |
| APA | 王家文.,武亿.,张潇.,张帆.,吕东媛.,...&Gao YX.(2019).Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation.STEM CELL RESEARCH & THERAPY,10(1),16. |
| MLA | 王家文,et al."Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation".STEM CELL RESEARCH & THERAPY 10.1(2019):16. |
入库方式： OAI收割
来源：力学研究所
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