Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands
文献类型：期刊论文
| 作者 | Shu XY(舒芯钰)1,2; 李宁1,2); Huang DD(黄丹丹)1,2; Zhang Y(章燕)1,2; Lv SQ(吕守芹)1,2; Long M(龙勉)1,2; Zhang Y(章燕) ; Zhang Y(章燕); Li N(李宁); Long M(龙勉)
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| 刊名 | EXPERIMENTAL CELL RESEARCH
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| 出版日期 | 2020-02-15 |
| 卷号 | 387期号:2页码:7 |
| 关键词 | beta(2) integrin Ligands Calcium transients Bond strength Mechanotransduction Shear stress |
| ISSN号 | 0014-4827 |
| DOI | 10.1016/j.yexcr.2019.111807 |
| 通讯作者 | Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) are key adhesion receptors to mediate neutrophil (PMN) recruitment and intracellular calcium (Ca2+) signaling. Binding of LFA-1 and Mac-1 to their ligands is essential in triggering Ca2+ transients and activating Ca2+-dependent kinases involved in cytoskeletal remodeling and migratory function. While mechanical forces are critical in regulating integrin-mediated Ca2+ transients, it is still unclear how the bond strength of beta(2)-integrin-ligand pair affects Ca2+ responses. Here three typical ligands with known mechanical features with LFA-1 and Mac-1 in our previous work were adopted to quantify their capabilities in inducing Ca2+ transients in adherent PMNs under shear flow. Data indicated that LFA-1 dominates Ca2+ transients in PMNs on intercellular adhesive molecule 1 (ICAM-1) and junctional adhesion molecule-A (JAM -A), while Mac-1 mediates Ca2+ transients induced by receptor for advanced glycation end products (RAGE), consistent with their corresponding bond strengths. These results link beta(2) integrin-ligand bond strength with Ca2+ transients in PMNs, suggesting high bond strength gives rise to strong Ca2+ response especially under physiological-like shear flow. The outcomes provide a new insight in understanding the mechanical regulatory mechanisms of PMN recruitment. |
| 分类号 | 二类 |
| WOS关键词 | JUNCTIONAL ADHESION MOLECULE ; FORCE ; ACTIVATION ; BINDING ; STRETCH ; ACCUMULATION ; PATHWAY ; INFLUX ; LFA-1 ; CELLS |
| 资助项目 | National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870930] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDB22040101] |
| WOS研究方向 | Oncology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000512482300015 |
| 资助机构 | National Natural Science Foundation of China ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences |
| 其他责任者 | Long, Mian |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/81592]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing 100190, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Shu XY,李宁1,2),Huang DD,et al. Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands[J]. EXPERIMENTAL CELL RESEARCH,2020,387(2):7. |
| APA | 舒芯钰.,李宁1,2).,黄丹丹.,章燕.,吕守芹.,...&Shu XY.(2020).Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands.EXPERIMENTAL CELL RESEARCH,387(2),7. |
| MLA | 舒芯钰,et al."Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands".EXPERIMENTAL CELL RESEARCH 387.2(2020):7. |
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来源：力学研究所
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