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  5. 期刊论文
Grass carp cGASL negatively regulates fish IFN response by targeting MITA

文献类型：期刊论文

作者Zhou, Yu1,4; Lu, Long-Feng4; Lu, Xiao-Bing1,4; Li, Shun4; Zhang, Yong-An2,3,4
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-11-01
卷号94页码:871-879
关键词cGAS-like Negative regulator Interferon MITA Grass carp
ISSN号1050-4648
DOI10.1016/j.fsi.2019.10.010
通讯作者Li, Shun(bob@ihb.ac.cn) ; Zhang, Yong-An(yzhang@ihb.ac.cn)
英文摘要Mammalian cyclic GMP-AMP synthase (cGAS) senses double-stranded (ds) DNA in the cytosol to activate the innate antiviral response. In the present study, a cGAS-like gene, namely cGASL, was cloned from grass carp Ctenopharyngodon idellus, and its role as a negative regulator of the IFN response was revealed. Phylogenetic analysis indicated that cGASL was evolutionarily closest to cGAS, but was not a true ortholog of cGAS. Overexpression of cGASL inhibited poly LC-stimulated grass carp (gc)IFN1pro and ISRE activities. In addition, MITA-, but not TBK1-mediated activation of gclFNlpro was impaired by cGASL. Co-immunoprecipitation and Western blot experiments indicated that cGASL interacted with MITA and TBK1, resulting in a reduction in the phosphorylation of MITA. Lastly, overexpression of cGASL reduced the transcriptional levels of several IFNstimulated genes activated by MITA. Collectively, these data suggest that cGASL is a negative regulator of IFN response by targeting MITA in fish.
WOS关键词GMP-AMP SYNTHASE ; DNA SENSOR CGAS ; RIG-I ; PROTEIN ; IRF3 ; REQUIREMENT ; EXPRESSION ; RECEPTORS ; PATHWAY ; MAB21L2
资助项目National Natural Science Foundation of China[31725026] ; National Natural Science Foundation of China[31502200] ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China[2018CFA011]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000496892400092
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China
源URL[http://ir.ihb.ac.cn/handle/342005/34797]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Li, Shun; Zhang, Yong-An
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Yu,Lu, Long-Feng,Lu, Xiao-Bing,et al. Grass carp cGASL negatively regulates fish IFN response by targeting MITA[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:871-879.
APA Zhou, Yu,Lu, Long-Feng,Lu, Xiao-Bing,Li, Shun,&Zhang, Yong-An.(2019).Grass carp cGASL negatively regulates fish IFN response by targeting MITA.FISH & SHELLFISH IMMUNOLOGY,94,871-879.
MLA Zhou, Yu,et al."Grass carp cGASL negatively regulates fish IFN response by targeting MITA".FISH & SHELLFISH IMMUNOLOGY 94(2019):871-879.

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来源：水生生物研究所

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