Identification and characterization of two mannan-binding lectin associated proteins in lectin complement pathway of grass carp
文献类型:期刊论文
作者 | Chen, Dan-Dan3,4; Yao, Yuan-Yuan1,4; Zhang, Yong-An2,4 |
刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY |
出版日期 | 2020-02-01 |
卷号 | 103期号:1页码:9 |
ISSN号 | 0145-305X |
关键词 | Lectin complement pathway Mannan-binding lectin-associated protein Competitive inhibition Grass carp |
DOI | 10.1016/j.dci.2019.103497 |
英文摘要 | The lectin pathway of complement activation is an important component of the innate immune response, which must be tightly controlled to maintain immune homeostasis. However, its control mechanisms have not been investigated in detail in bony fish. In this study, we identified and characterized two novel, phylogenetically conserved mannan-binding lectin (MBL)-associated proteins (MAps) of grass carp (Ctenopharyngodon idella), CiMAp27 and CiMAp39, which were truncated, alternatively-spliced forms of grass carp MBL-associated serine proteases (MASPs), CiMASP1 and CiMASP2, respectively. Gene expression profiling showed that both CiMAp27 and CiMAp39 were upregulated by low doses of Aeromonas hydrophila, and inhibited by high doses, which lead to the inference that these genes acted as immune factors in antibacterial defense. Sequence analysis showed that CiMAp27 lack a catalytic domain but retains two domains (CUB1-EGF) involved in the association with MBL, while CiMAp39 retained four domains (CUB1-EGF-CUB2-CCP1). Not only the two CiMASPs but also the CiMAps were detected in grass carp serum. Furthermore, both recombinant CiMASPs (rCiMASPs) and recombinant rCiMAps (rCiMAps) interacted with recombinant MBL and the two CiMAps competed with CiMASPs for binding to MBL, and hence inhibited downstream C4 binding. These results indicated that CiMAps acted as competitive inhibitors in the lectin complement pathway of grass carp. |
WOS关键词 | SERINE-PROTEASE (MASP)-1 ; IMMUNE-RESPONSES ; ACTIVATION ; MASP-1 ; SYSTEM ; MBL ; MECHANISM ; AND-2 |
资助项目 | National Natural Science Foundation of China[31725026] ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China[2018CFA011] |
WOS研究方向 | Fisheries ; Immunology ; Zoology |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000500050700003 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China ; 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源URL | [http://ir.ihb.ac.cn/handle/342005/34853] |
专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
通讯作者 | Zhang, Yong-An |
作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Dan-Dan,Yao, Yuan-Yuan,Zhang, Yong-An. Identification and characterization of two mannan-binding lectin associated proteins in lectin complement pathway of grass carp[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,103(1):9. |
APA | Chen, Dan-Dan,Yao, Yuan-Yuan,&Zhang, Yong-An.(2020).Identification and characterization of two mannan-binding lectin associated proteins in lectin complement pathway of grass carp.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,103(1),9. |
MLA | Chen, Dan-Dan,et al."Identification and characterization of two mannan-binding lectin associated proteins in lectin complement pathway of grass carp".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 103.1(2020):9. |
入库方式: OAI收割
来源:水生生物研究所
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