中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase

文献类型:期刊论文

作者Zhao, Yu-Han2; Zeng, Xiao-Tao2; Zhang, Qi-Ya1,2
刊名VIRUS RESEARCH
出版日期2020-01-02
卷号275页码:7
ISSN号0168-1702
关键词Carassius auratus herpesvirus (CaHV) Viral protein Mitochondrial FoF1-ATPase Virus-host interaction Yeast two hybrid (Y2H) Co-immunoprecipitation (co-IP) assays
DOI10.1016/j.virusres.2019.197754
通讯作者Zhang, Qi-Ya(zhangqy@ihb.ac.cn)
英文摘要Herpesvirus infection usually relies on the interaction between viral protein and host protein to enhance replication of the enveloped virus. Fish Carassius auratus herpesvirus (CaHV) is highly pathogenic pathogen causing gill acute hemorrhages of crucian carp (Carassius auratus) and high moritality rates among those infected fish. The protein of CaHV (CaHV-138 L) containing two transmembrane (TM) domains and an immunoglobulin C-2 Type (IGc2) domain was predicted as a viral membrane protein. In this investigation, fluorescence observation showed that full-length CaHV-138 L mainly localized on the plasma membrane or around nuclear membrane of fish fathead minnow (FHM) cells in a punctate pattern. The TM domain deletion mutants of CaHV-138 L (Delta TM1, Delta TM2, and Delta TM1 &Delta TM2) diffusely distributed in both the cytoplasm and the nucleus, mainly presented patchy fashion in the cytoplasm, and mainly presented both in the nucleus and in the cytoplasm, respectively. Obviously, the TM domain deletion mutants significantly affected CaHV-138 L subcellular localization. Meanwhile, colocalization assay showed that the full-length viral protein colocalized with mitochondria. Furthermore, the interaction between CaHV-138 L and host protein was identified by yeast two-hybrid (Y2H) and co-immunoprecipitation (co-IP) assays. The host mitochondrial protein FoF1 ATP synthase (FoF1-ATPase) that interacts with this viral protein was screened. The data indicated that CaHV-138 L can target to mitochondrial protein FoF1-ATPase, which might provide energy for virus replication through mediating mitochondrial ATP synthesis. This study has provided valuable information for better understanding of the links of herpesvirus proteins with aquaculture animal proteins.
WOS关键词CARASSIUS-AURATUS HERPESVIRUS ; MOLECULAR-BASIS ; MEMBRANE ; SYNTHASE ; REVEALS
资助项目National Natural Science Foundation of China[31772890] ; National Natural Science Foundation of China[31430091] ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project[XDA08030202] ; National Key R&D Plan of the Ministry of Science and Technology, China[2018YFD0900302]
WOS研究方向Virology
语种英语
出版者ELSEVIER
WOS记录号WOS:000500373700003
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China ; National Key R&D Plan of the Ministry of Science and Technology, China
源URL[http://ir.ihb.ac.cn/handle/342005/34806]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Zhang, Qi-Ya
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式
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Zhao, Yu-Han,Zeng, Xiao-Tao,Zhang, Qi-Ya. Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase[J]. VIRUS RESEARCH,2020,275:7.
APA Zhao, Yu-Han,Zeng, Xiao-Tao,&Zhang, Qi-Ya.(2020).Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase.VIRUS RESEARCH,275,7.
MLA Zhao, Yu-Han,et al."Fish herpesvirus protein (CaHV-138L) can target to mitochondrial protein FoF1 ATPase".VIRUS RESEARCH 275(2020):7.

入库方式: OAI收割

来源:水生生物研究所

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