Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53
文献类型：期刊论文
| 作者 | Guan, Xiao-lu2,3; Zhang, Bao-cun1,4; Sun, Li2,3 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2020-02-01 |
| 卷号 | 103页码:8 |
| ISSN号 | 0145-305X |
| 关键词 | miRNA Paralichthys olivaceus Autophagy Bacterial infection Immune defense |
| DOI | 10.1016/j.dci.2019.103531 |
| 通讯作者 | Sun, Li(Isun@qdio.ac.cn) |
| 英文摘要 | MicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3'UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda. |
| 资助项目 | National Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000500050700027 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/163747]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Aarhus Univ, Aarhus Res Ctr Innate Immun, Aarhus, Denmark 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Aarhus Univ, Dept Biomed, Aarhus, Denmark |
| 推荐引用方式 GB/T 7714 | Guan, Xiao-lu,Zhang, Bao-cun,Sun, Li. Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,103:8. |
| APA | Guan, Xiao-lu,Zhang, Bao-cun,&Sun, Li.(2020).Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,103,8. |
| MLA | Guan, Xiao-lu,et al."Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 103(2020):8. |
入库方式： OAI收割
来源：海洋研究所
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