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Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis

文献类型：期刊论文

作者Du, Yang4,5; Fang, Han3,4; Shao, Xuqing1; Liu, Mei4,5; Jiang, Keyong4,5; Wang, Mengqiang4,5; Wang, Baojie4,5; Wang, Lei2,4,5
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-12-01
卷号95页码:368-382
关键词Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Proteomic analysis
ISSN号1050-4648
DOI10.1016/j.fsi.2019.10.059
通讯作者Wang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(wanglei@qdio.ac.cn)
英文摘要Our previous work showed that using Lactobacillus pentosus HC-2 as a probiotic could improve the growth performance, immune response, gut bacterial diversity and disease resistance of Litopenaeus vannamei. However, the probiotic mechanism had not been fully characterized. In the present study, histology and proteomic analysis were performed to explore the influence of HC-2 surface protein on its probiotic effects on L. vannamei after feeding either the intact surface proteins, the probiotic treated with lithium chloride (LiCl) to remove noncovalently bound surface proteins or no probiotic for four weeks. Histological observation found that feeding with normal HC-2 obviously improved the intestinal histology and enhanced the protective effect against pathogen damage, but feeding with LiCl-treated HC-2 did not improve the intestinal environment. A total of over 2764 peptides and 1118 uniproteins were identified from the L. vannamei midgut; 211 proteins were significantly differentially expressed in the normal HC-2 group compared with the control group; 510 proteins were significantly differentially expressed in the LiCl-treated HC-2 group compared with the control group, and 458 proteins were significantly differentially expressed in the LiCl-treated HC-2 group compared with the normal HC-2 group. GO/KEGG enrichment analysis of the significantly different proteins demonstrated that feeding normal HC-2 mainly induced immune response, metabolic, cell adhesion and cell-cell signaling-related protein upregulation, which contributed to bacterial adhesion and colonization in the midgut to improve the shrimp immune system and growth, but these proteins were suppressed after the shrimp were fed bacteria deprived of surface proteins. Taken together, these results indicate that the surface proteins were indispensable for HC-2 to execute probiotic effects in the shrimp midgut.
资助项目National Natural Science Foundation of China[41906107] ; China Postdoctoral Science Foundation[2018M632736] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000503099200036
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://ir.qdio.ac.cn/handle/337002/164109]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Wang, Baojie; Wang, Lei
作者单位1.Shandong Cigna Detect Technol Co Ltd, Qingdao 266237, Shandong, Peoples R China
2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Du, Yang,Fang, Han,Shao, Xuqing,et al. Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:368-382.
APA Du, Yang.,Fang, Han.,Shao, Xuqing.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis.FISH & SHELLFISH IMMUNOLOGY,95,368-382.
MLA Du, Yang,et al."Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis".FISH & SHELLFISH IMMUNOLOGY 95(2019):368-382.

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来源：海洋研究所

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