Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Ji, Li-Qin2,3; Rao, Ying-Zhu1,2; Zhang, Yong4; Chen, Rong1; Tao, Ya-Xiong2 |
| 刊名 | GENERAL AND COMPARATIVE ENDOCRINOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 285页码:11 |
| 关键词 | Epinephelus coioides Orange-spotted grouper Melanocortin-1 receptor Melanocortin receptor accessory protein 2 Tissue distribution Pharmacology |
| ISSN号 | 0016-6480 |
| DOI | 10.1016/j.ygcen.2019.113291 |
| 通讯作者 | Tao, Ya-Xiong(taoyaxi@auburn.edu) |
| 英文摘要 | Melanocortin-1 receptor (MC1R) has important roles in regulating pigmentation and inflammation. Melanocortin receptor accessory protein 2 (MRAP2) modulates trafficking, ligand binding, and signaling of mammalian melanocortin receptors. However, the effect of MRAP2 on fish MC1R has not been extensively studied. Herein, we cloned the orange-spotted grouper (Epinephelus coioides) mc1r, which had a 972 bp open reading frame encoding a putative protein of 323 amino acids. Grouper mclr was mainly expressed in the brain, skin, testis, spleen, head kidney, and kidney. EcoMC1R showed high constitutive activities in both Gs-cAMP and ERK1/2 pathways, which could be differentially modulated by grouper MRAP2 (EcoMRAP2). Three agonists, including alpha-melanocyte-stimulating hormone (MSH), beta-MSH, and ACTH, could bind to EcoMC1R and dose-dependently increase intracellular cAMP production. EcoMRAP2 had no effect on the IC50 in binding assay or EC50 in cAMP assay; however, it dose-dependently decreased the cell surface expression and maximal response to the three agonists. EcoMRAP2 increased basal ERK1/2 activation but did not alter alpha-MSH-stimulated ERK1/2 activation. This study extends the knowledge base of fish MC1R pharmacology and its regulation by MRAP2. |
| 资助项目 | Science and Technology Planning Project of Guangdong Province[2014A020208136] ; Science and Technology Planning Project of Zhanjiang[2018A03027] ; Key Science Project of Lingnan Normal University[LZL1508] ; Guangdong Provincial Natural Science Foundation[20188030311026] ; Guangdong Provincial Science and Technology Program[2017B0202450001] ; Guangdong Provincial Science and Technology Program[2017B090904022] ; Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams ; Special Fund for Fisheries -Scientific Research of Guangdong Province[SDYY-2018-04] ; Science and Technology Planning Project of Guangzhou[201804020013] ; Independent Project of Guangdong Province Laboratory[ZJW-2019-06] ; Overseas Scholarship Program for Elite Young and Middle-aged Teachers of Lingnan Normal University ; Auburn University Animal Health and Disease Research Program of College of Veterinary Medicine at Auburn University ; China Scholarship Council, People's Republic of China |
| WOS研究方向 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000504341800020 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/164130]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Tao, Ya-Xiong |
| 作者单位 | 1.Lingnan Normal Univ, Life Sci & Technol Sch, Inst Appl Biotechnol, Zhanjiang 524048, Guangdong, Peoples R China 2.Auburn Univ, Coll Vet Med, Dept Anat Physiol & Pharmacol, Auburn, AL 36849 USA 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 4.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Southern Lab Ocean Sci & Engn Zhuhai, Zhuhai 51900, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Li-Qin,Rao, Ying-Zhu,Zhang, Yong,et al. Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2020,285:11. |
| APA | Ji, Li-Qin,Rao, Ying-Zhu,Zhang, Yong,Chen, Rong,&Tao, Ya-Xiong.(2020).Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides).GENERAL AND COMPARATIVE ENDOCRINOLOGY,285,11. |
| MLA | Ji, Li-Qin,et al."Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 285(2020):11. |
入库方式： OAI收割
来源：海洋研究所
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