Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine
文献类型：期刊论文
| 作者 | Yang, Yue1,2,3; Xing, Ronge1,3 ; Liu, Song1,3; Qin, Yukun1,3; Li, Kecheng1,3; Yu, Huahua1,3; Li, Pengcheng1,3
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2020-02-01 |
| 卷号 | 229页码:9 |
| ISSN号 | 0144-8617 |
| 关键词 | Newcastle disease virus Adjuvant Nanoparticle Chitosan Hydroxypropyltrimethyl ammonium chloride chitosan Sulfated chitosan |
| DOI | 10.1016/j.carbpol.2019.115423 |
| 通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
| 英文摘要 | Chitosan (CS) and its water-soluble derivatives, hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and sulfated chitosan (SCS), were used as adjuvants of inactivated Newcastle disease (ND) vaccine. First, NDV-loaded and blank CS, HACC/CS and SCS nanoparticles were prepared. The particle sizes were respectively 343.43 +/- 4.12, 320.03 +/- 0.84, 156.2 +/- 9.29 nm and the zeta potentials were respectively +19.67 +/- 0.58, +18.3 +/- 0.5, -17.8 +/- 2.65 mV under the optimal conditions. Then chickens were immunized with nanoparticles or commercial inactivated oil emulsion vaccine. After immunization, the humoral immunity levels of the chickens were evaluated. The cellular immunity levels were determined by the quantification of cytokines, lymphocyte proliferation assay, the percentages of CD4(+) and CD8(+) T lymphocytes. Finally, the chickens were challenged with highly virulent virus. The results demonstrated that the humoral immunity levels in NDV-loaded CS and HACC/CS nanoparticles groups were lower than commercial vaccine but the cellular immunity levels are better. Moreover, the prevention effects of NDV-loaded CS and HACC/CS nanoparticles against highly virulent NDV are comparable to commercial vaccine. Our study provides the basis of developing HACC and CS as effective vaccine adjuvants. |
| 资助项目 | National Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Special projects of Foshan science and technology innovation team[2017IT100054] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000501796600026 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/164169]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xing, Ronge; Li, Pengcheng |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yue,Xing, Ronge,Liu, Song,et al. Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine[J]. CARBOHYDRATE POLYMERS,2020,229:9. |
| APA | Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2020).Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine.CARBOHYDRATE POLYMERS,229,9. |
| MLA | Yang, Yue,et al."Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine".CARBOHYDRATE POLYMERS 229(2020):9. |
入库方式： OAI收割
来源：海洋研究所
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