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Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei

文献类型：期刊论文

作者Guo, Qing6; Li, Shihao1,2,6; Lv, Xinjia3,6; Xiang, Jianhai1,6; Manor, Rivka4,5; Sagi, Amir4,5; Li, Fuhua1,2,6
刊名FRONTIERS IN PHYSIOLOGY
出版日期2019-12-20
卷号10页码:14
关键词CHH "eyestalk-AG-testis" endocrine axis guanylate cyclase IAG male sexual differentiation
ISSN号1664-042X
DOI10.3389/fphys.2019.01525
通讯作者Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cnl)
英文摘要The "eyestalk-androgenic gland (AG)-testis" endocrine axis is involved in male sexual differentiation of crustaceans. The insulin-like androgenic gland hormone (IAG), secreted from the AG, plays a central role in this axis, however key factors upstream the IAG are still poorly understood. Here, two crustacean hyperglycemic hormone (CHH) genes (LvCHH1 and LvCHH2) and their putative receptor guanylate cyclase (LvGC) were identified in Litopenaeus vannamei. LvCHH1 and LvCHH2 belonged to CHH subfamily I members and LvGC was a membrane-bound guanylate cyclase. They were all differentially expressed in eyestalks and gonads of males and females. RNA interference (RNAi) of either LvCHH1 or LvCHH2 increased LvIAG expression, while injection of their recombinant protein decreased LvIAG expression, indicating that LvCHH1 and LvCHH2 are inhibitory factors of LvIAG expression. Yeast two-hybrid assay showed that both LvCHH1 and LvCHH2 interacted with LvGC and their RNAi and recombinant protein injection exerted opposite regulatory effects on the transcriptional expression of LvGC. Meanwhile, knockdown of LvGC increased LvIAG expression. These results suggest that LvGC is the receptor of LvCHH1 and LvCHH2 and they are all involved in male sexual development by regulating LvIAG expression. The present study unveils missing upstream elements in the "eyestalk-AG-testis" endocrine axis in crustacean.
资助项目National Key Research and Development Program of China[2018YFD0900502] ; National Natural Science Foundation of China[31861143047] ; ISF-NSFC Joint Research Program[2368/18] ; China Agriculture Research System-48[CARS-48]
WOS研究方向Physiology
语种英语
WOS记录号WOS:000505705400001
出版者FRONTIERS MEDIA SA
源URL[http://ir.qdio.ac.cn/handle/337002/164202]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Shihao; Li, Fuhua
作者单位1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel
5.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel
6.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Qing,Li, Shihao,Lv, Xinjia,et al. Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei[J]. FRONTIERS IN PHYSIOLOGY,2019,10:14.
APA Guo, Qing.,Li, Shihao.,Lv, Xinjia.,Xiang, Jianhai.,Manor, Rivka.,...&Li, Fuhua.(2019).Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei.FRONTIERS IN PHYSIOLOGY,10,14.
MLA Guo, Qing,et al."Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei".FRONTIERS IN PHYSIOLOGY 10(2019):14.

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来源：海洋研究所

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