p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis
文献类型：期刊论文
| 作者 | Zhang, Shujing2,3,4; Yu, Jiajia2,3 ; Wang, Hongxia1,3; Liu, Baozhong1,2,3; Yue, Xin1,3
|
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2019-12-01 |
| 卷号 | 95页码:456-463 |
| 关键词 | p38 MAPK Vibrio challenge Immune defence MITF |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.10.048 |
| 通讯作者 | Yue, Xin(yuexin@qdio.ac.cn) |
| 英文摘要 | p38 mitogen-activated protein kinases (MAPKs) are involved in the response to various extracellular stimuli via regulating gene expression. In the present study, a p38 MAPK gene (MpP38) was identified from the clam Meretrix petechialis. The full-length cDNA of MpP38 measures 1,720 bp, consisting of a 134-bp 5'-UTR, a 1,095-bp ORF and a 491-bp 3'-UTR. Both the mRNA and protein expression levels of MpP38 increased after Vibrio challenge, implying that MpP38 is involved in clam immunity. Based on our previous study, a transcription factor activated by p38 MAPK, i.e. microphthalmia-associated transcription factor (MITF), participated in clam immunity by regulating the expression of phenoloxidase (PO). Coupled with other related reports, the mechanism underlying the involvement of MpP38 in clam immunity is most likely that pathogen stimuli induce the phosphorylation of p38 MAPK and thus activate MITF to regulate the expression of the immune-related gene PO. The results obtained in this study support this mechanism. First, we found that the MpP38 phosphorylation level increased in response to Vibrio challenge. Second, as revealed by a yeast two-hybrid assay, there was a direct interaction between MpP38 and MITF. Meanwhile, inhibiting the phosphorylation of MpP38 decreased the phosphorylation level of MpMITF, implying that MpP38 phosphorylation is required for MpMITF activation. Additionally, our results showed that there was a regulatory relationship between MpP38 phosphorylation level and PO expression level. With increased MpP38 phosphorylation level, the PO expression level was also increased after Vibrio challenge; when MpP38 phosphorylation was inhibited, the PO expression level was significantly decreased. This study describes the immune function of p38 MAPK in the clam for the first time and analyses its potential underlying mechanism, which will help to elucidate the immune mechanism in the clam M. petechialis. |
| 资助项目 | Marine S&T Fund of Shandong Province (China)[2018SDKJ0502-1] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology (China)[YQ2018N007] ; Agriculture Research System of China[CARS-49] ; National Key R&D Program of China[2019YFD0900700] ; National Natural Science Foundation of China[NSFC 31772845] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000503099200044 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/164358]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yue, Xin |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Shandong Univ Technol, Sch Life Sci, Antiaging & Regenerat Med Res Inst, Shandong Prov Key Lab Anim Cell & Dev Biol, Zibo 255049, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shujing,Yu, Jiajia,Wang, Hongxia,et al. p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:456-463. |
| APA | Zhang, Shujing,Yu, Jiajia,Wang, Hongxia,Liu, Baozhong,&Yue, Xin.(2019).p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,95,456-463. |
| MLA | Zhang, Shujing,et al."p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 95(2019):456-463. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。