Reductive degradation of chloramphenicol by Geobacter metallireducens
文献类型：期刊论文
| 作者 | Xu HengDuo2,4; Xiao LeiLei2; Zheng ShiLing2; Zhang YueChao1,2,3; Li JiaJia1,2,3; Liu FangHua2,3 |
| 刊名 | SCIENCE CHINA-TECHNOLOGICAL SCIENCES
 |
| 出版日期 | 2019-10-01 |
| 卷号 | 62期号:10页码:1688-1694 |
| 关键词 | chloramphenicol Geobacter metallireducens dechlorination extracellular electron transfer reduction |
| ISSN号 | 1674-7321 |
| DOI | 10.1007/s11431-018-9415-2 |
| 通讯作者 | Liu FangHua(fhliu@yic.ac.cn) |
| 英文摘要 | Geobacter metallireducens is known to be capable of removing nitroaromatic compounds via an oxidation mode. However, little attention has been paid to investigate the reductive removal of chlorinated nitroaromatic compounds by G. metallireducens. In this study, G. metallireducens was used to reduce chloramphenicol (CAP), a typical chlorinated nitroaromatic antibiotic. Cyclic voltammograms and chronoamperometry highlighted a higher peak current for CAP reduction by G. metallireducens compared to the control without bacteria. G. metallireducens efficiently reduced CAP (20 mg/L) with acetate as the sole electron donor, and the removal efficiency reached (97.6 +/- 4.9)% within 6 d. Aromatic amine (AMCl2), AMCl (dechlorinated AMCl2) and AM (dechlorinated AMCl) were identified as reduction products by liquid chromatography-mass spectrometry. However, the removal efficiency declined to (25.0 +/- 3.6)% when the CAP dosage increased to 80 mg/L. Transcriptomic analysis indicated the significant upregulation of genes related to electron transfer, such as pilus assembly protein gene (2.8 folds), NADH-quinone oxidoreductase subunit K2 gene (4.5 folds) and many c-type cytochrome genes such as cytochrome c biogenesis protein ResB (Gmet 2901, 4.6 folds), cytochrome c (Gmet 0335, 4.4 folds) and cytochrome c7 (Gmet 2902, 3.4 folds). Furthermore, a gene related to chlorinated contaminant removal (Gmet 1046, 5.4 folds) was also upregulated, possibly resulting in enhanced CAP reduction. This work deepened our knowledge of the bioremediation ability of G. metallireducens with respect to environmental contaminants and provided a potential strategy to treat antibiotics with electrochemically active bacteria. |
| 资助项目 | Postdoctoral Science Foundation of China[2018M632735] ; National Natural Science Foundation of China[91751112] ; National Natural Science Foundation of China[41573071] ; Senior User Project of RV KEXUE[KEXUE2018G01] ; Natural Science Foundation[JQ201608] ; Young Taishan Scholars Program of Shandong Province[tsqn20161054] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000504097800004 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/164229]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Liu FangHua |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu HengDuo,Xiao LeiLei,Zheng ShiLing,et al. Reductive degradation of chloramphenicol by Geobacter metallireducens[J]. SCIENCE CHINA-TECHNOLOGICAL SCIENCES,2019,62(10):1688-1694. |
| APA | Xu HengDuo,Xiao LeiLei,Zheng ShiLing,Zhang YueChao,Li JiaJia,&Liu FangHua.(2019).Reductive degradation of chloramphenicol by Geobacter metallireducens.SCIENCE CHINA-TECHNOLOGICAL SCIENCES,62(10),1688-1694. |
| MLA | Xu HengDuo,et al."Reductive degradation of chloramphenicol by Geobacter metallireducens".SCIENCE CHINA-TECHNOLOGICAL SCIENCES 62.10(2019):1688-1694. |
入库方式： OAI收割
来源：海洋研究所
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