中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine

文献类型:期刊论文

作者Chen, Chao1,2,3,4; Wang, Yi2,3,4; Zhang, Dun2,3,4
刊名MICROCHIMICA ACTA
出版日期2019-12-01
卷号186期号:12页码:8
关键词VS4 Catalytic TMB Mechanism Nanozyme Hydroxyl radical Colorimetric Detection H2O2 Transition metal chalcogenides
ISSN号0026-3672
DOI10.1007/s00604-019-3942-3
通讯作者Wang, Yi(wangyi@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要It is demonstrated that vanadium tetrasulfide (VS4) exhibits peroxidase (POx)-like activity which follows Michaelis-Menten kinetics when using H2O2 as a co-substrate. Electron spin resonance spectroscopy was use to analyze the catalytic mechanism. It suggests that the enzyme mimicking activity is caused by decomposing H2O2 into hydroxyl radicals. The method was used to quantify H2O2 by using 3,3 ',5,5 '-tetramethylbenzidine as the substrate which results in the formation of a blue coloration (with an absorption peak at 652 nm). H2O2 can be detected in the 50 to 300 mu M concentration range, and the detection limit is 5.0 mu M. The assay for L-cysteine (L-cys) is based on the capability of oxTMB to oxidize L-cys to form L-cystine. The colorimetric L-cys assay has a linear response in the 5 to 100 mu M concentration range and a 2.5 mu M detection limit. Graphical abstract Schematic representation of the enzyme mimicking activity of vanadium tetrasulfide (VS4) submicrospheres originated from the decomposition of hydrogen peroxide (H2O2) to generate reactive hydroxyl radical (center dot OH) and the colorimetric detection of L-cysteine.
资助项目Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050104] ; Natured Science Foundation of China[41776090] ; Key Research and Development Program of Shandong Province[2018GHY115038] ; Key Research and Development Program of Shandong Province[2018GGX104021] ; AoShan Talent Program ; Qingdao National Laboratory for Marine Science and Technology
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000498188200010
出版者SPRINGER WIEN
源URL[http://ir.qdio.ac.cn/handle/337002/163792]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Wang, Yi; Zhang, Dun
作者单位1.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Chen, Chao,Wang, Yi,Zhang, Dun. Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine[J]. MICROCHIMICA ACTA,2019,186(12):8.
APA Chen, Chao,Wang, Yi,&Zhang, Dun.(2019).Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine.MICROCHIMICA ACTA,186(12),8.
MLA Chen, Chao,et al."Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine".MICROCHIMICA ACTA 186.12(2019):8.

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来源:海洋研究所

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