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  5. 微观重建与智能分析
Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution

文献类型：期刊论文

作者Wang, Hao2,3,4; Zhu, Qingyuan2,3; Ding, Lufeng3,4; Shen, Yan3,4; Yang, Chao-Yu3,4; Xu, Fang3,4; Shu, Chang1,5; Guo, Yujie2,3; Xiong, Zhiwei6,7; Shan, Qinghong3,4
刊名NATIONAL SCIENCE REVIEW
出版日期2019-09-01
卷号6期号:5页码:982-992
ISSN号2095-5138
关键词fluorescence microscopy brain mapping tissue clearing immunostaining activity trace mapping
DOI10.1093/nsr/nwz053
通讯作者Zhu, Qingyuan(qyzhu@ustc.edu.cn) ; Lau, Pak-Ming(plau@ustc.edu.cn) ; Bi, Guo-Qiang(gqbi@ustc.edu.cn)
英文摘要The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits, which is of fundamental importance for neuroscience research. Here, we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout (VISoR) for high-throughput, high-quality brain mapping. Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion, the VISoR system effectively eliminates motion blur to obtain undistorted images. By continuously imaging moving samples without stopping, the system achieves high-speed 3D image acquisition of an entire mouse brain within 1.5 hours, at a resolution capable of visualizing synaptic spines. A pipeline is developed for sample preparation, imaging, 3D image reconstruction and quantification. Our approach is compatible with immunofluorescence methods, enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains. Using this system, we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-shEGFP mice under resting or forced swimming conditions. Our results indicate the involvement of multiple subcortical areas in stress response. Intriguingly, neuronal activation in these areas exhibits striking individual variability among different animals, suggesting the necessity of sufficient cohort size for such studies.
WOS关键词SELECTIVE PLANE ILLUMINATION ; SEMLIKI-FOREST-VIRUS ; MOUSE ; NEURONS ; TOMOGRAPHY ; MICROSCOPY ; NETWORKS ; TISSUE ; RANGE ; MICE
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02050000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02060000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB02030000] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences[YZ201668] ; National Natural Science Foundation of China[91732304] ; National Natural Science Foundation of China[91632303] ; National Natural Science Foundation of China[91432305] ; National Basic Research Program of China[2013CB835101] ; Fundamental Research Funds for the Central Universities[WK6030000033]
WOS研究方向Science & Technology - Other Topics
语种英语
出版者OXFORD UNIV PRESS
WOS记录号WOS:000512966500023
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; Scientific Instrument Developing Project of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Basic Research Program of China ; Fundamental Research Funds for the Central Universities
源URL[http://ir.ia.ac.cn/handle/173211/28578]  
专题类脑智能研究中心_微观重建与智能分析
通讯作者Zhu, Qingyuan; Lau, Pak-Ming; Bi, Guo-Qiang
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
4.Univ Sci & Technol China, CAS Key Lab Brain Funct & Dis, Hefei 230027, Peoples R China
5.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
6.Univ Sci & Technol China, Sch Informat Sci & Technol, Hefei 230027, Peoples R China
7.Univ Sci & Technol China, Natl Engn Lab Brain Inspired Intelligence Technol, Hefei 230027, Peoples R China
8.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
9.CAS Ctr Excellence Brain Sci & Intelligence Techo, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Hao,Zhu, Qingyuan,Ding, Lufeng,et al. Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution[J]. NATIONAL SCIENCE REVIEW,2019,6(5):982-992.
APA Wang, Hao.,Zhu, Qingyuan.,Ding, Lufeng.,Shen, Yan.,Yang, Chao-Yu.,...&Bi, Guo-Qiang.(2019).Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution.NATIONAL SCIENCE REVIEW,6(5),982-992.
MLA Wang, Hao,et al."Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution".NATIONAL SCIENCE REVIEW 6.5(2019):982-992.

入库方式： OAI收割

来源：自动化研究所

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