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In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT

文献类型：期刊论文

作者Huang, Wenhui5,6; Wang, Kun6; An, Yu6; Meng, Hui6; Gao, Yuan6; Xiong, Zhiyuan5,7; Yan, Hao4; Wang, Qian3; Cai, Xuekang2; Yang, Xin6
刊名EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
出版日期2019-11-08
页码12
关键词Nasopharyngeal carcinoma Tumour hypoxia Fluorescence molecular tomography Multispectral optoacoustic tomography Carbonic anhydrase IX
ISSN号1619-7070
DOI10.1007/s00259-019-04526-x
通讯作者Yang, Xing(yangxing2017@bjmu.edu.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Zhang, Shuixing(shui7515@126.com)
英文摘要Purpose Accurate evaluation of hypoxia is particularly important in patients with nasopharyngeal carcinoma (NPC) undergoing radiotherapy. The aim of this study was to propose a novel imaging strategy for quantitative three-dimensional (3D) evaluation of hypoxia in a small animal model of NPC. Methods A carbonic anhydrase IX (CAIX)-specific molecular probe (CAIX-800) was developed for imaging of hypoxia. Mouse models of subcutaneous, orthotopic, and spontaneous lymph node metastasis from NPC (5 mice per group) were established to assess the imaging strategy. A multi-modality imaging method that consisted of a hybrid combination of fluorescence molecular tomography-computed tomography (FMT-CT) and multispectral optoacoustic tomography (MSOT) was used for 3D quantitative evaluation of tumour hypoxia. Magnetic resonance imaging, histological examination, and immunohistochemical analysis were used as references for comparison and validation. Results In the early stage of NPC (2 weeks after implantation), FMT-CT enabled precise 3D localisation of the hypoxia biomarker with high sensitivity. At the advanced stage (6 weeks after implantation), MSOT allowed multispectral analysis of the biomarker and haemoglobin molecules with high resolution. The combination of high sensitivity and high resolution from FMT-CT and MSOT could not only detect hypoxia in small-sized NPCs but also visualise the heterogeneity of hypoxia in 3D. Conclusions Integration of FMT-CT and MSOT could allow comprehensive and quantifiable evaluation of hypoxia in NPC. These findings may potentially benefit patients with NPC undergoing radiotherapy in the future.
WOS关键词CARBONIC-ANHYDRASE-IX ; FLUORESCENCE MOLECULAR TOMOGRAPHY ; COMBINING ANATOMY ; CELL CARCINOMA ; EXPRESSION ; HEAD ; CANCER ; MODEL ; PET ; CARBONIC-ANHYDRASE-9
资助项目Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2015CB755500] ; National Natural Science Foundation of China[61671449] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81571664] ; National Natural Science Foundation of China[81871323] ; National Natural Science Foundation of China[81801665] ; National Natural Science Foundation of China[21877004] ; National Thousand Young Talents Program of China ; Chinese Academy of Sciences[GJJSTD20170004] ; Chinese Academy of Sciences[KFJ-STS-ZDTP-059] ; Chinese Academy of Sciences[YJKYYQ20180048] ; National Natural Science Foundation of Guangdong Province[2018B030311024] ; Scientific Research General Project of Guangzhou Science Technology and Innovation Commission Grant[201707010328] ; China Postdoctoral Science Foundation[2016 M600145] ; Clinical Medicine Plus X-Young Scholars Project, Peking University, the Fundamental Research Funds for the Central Universities
WOS研究方向Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:000495208500003
出版者SPRINGER
资助机构Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Thousand Young Talents Program of China ; Chinese Academy of Sciences ; National Natural Science Foundation of Guangdong Province ; Scientific Research General Project of Guangzhou Science Technology and Innovation Commission Grant ; China Postdoctoral Science Foundation ; Clinical Medicine Plus X-Young Scholars Project, Peking University, the Fundamental Research Funds for the Central Universities
源URL[http://ir.ia.ac.cn/handle/173211/28900]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Yang, Xing; Tian, Jie; Zhang, Shuixing
作者单位1.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing, Peoples R China
2.Peking Univ, Dept Nucl Med, Hosp 1, 8 Xishiku Rd, Beijing 100034, Peoples R China
3.Chinese Acad Med Sci & Peking Union Med Coll, Dept Diagnost Imaging, Natl Canc Ctr, Canc Hosp, Beijing 100021, Peoples R China
4.Tsinghua Univ, Engn Lab Functionalized Carbon Mat, Grad Sch Shenzhen, Shenzhen 518055, Peoples R China
5.Jinan Univ, Affiliated Hosp 1, Med Imaging Ctr, 163 Huangpu West Rd, Guangzhou 510632, Guangdong, Peoples R China
6.Chinese Acad Sci, CAS Key Lab Mol Imaging, Inst Automat, 95 Zhongguancun East Rd, Beijing 100190, Peoples R China
7.Univ Melbourne, Dept Chem & Biomol Engn, Melbourne, Vic 3010, Australia
推荐引用方式
GB/T 7714		 Huang, Wenhui,Wang, Kun,An, Yu,et al. In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT[J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,2019:12.
APA Huang, Wenhui.,Wang, Kun.,An, Yu.,Meng, Hui.,Gao, Yuan.,...&Zhang, Shuixing.(2019).In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING,12.
MLA Huang, Wenhui,et al."In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT".EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING (2019):12.

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来源：自动化研究所

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