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Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing

文献类型：期刊论文

作者Chang MY6,7; Liu J6; Guo BL7; Fang X(方新)5; Wang Y7; Wang SY4,7; Liu XF3; Reid LM1,2; Wang YF6,7
刊名FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
出版日期2020-02-21
卷号8页码:16
关键词cell delivery auto micro atomization device (AMAD) cell therapy wound healing organoids
ISSN号2296-4185
DOI10.3389/fbioe.2020.00110
通讯作者Wang, Yunfang(wangyf1972@gmail.com)
英文摘要Severe skin wounds are often associated with large areas of damaged tissue, resulting in substantial loss of fluids containing electrolytes and proteins. The net result is a vulnerability clinically to skin infections. Therapies aiming to close these large openings are effective in reducing the complications of severe skin wounds. Recently, cell transplantation therapy showed the potential for rapid re-epithelialization of severe skin wounds. Here, we show the improved effects of cell transplantation therapy using a robust protocol of efficient expansion and delivery of epidermal cells for treatment of severe skin wounds. Human skin tissues were used to generate human epidermal organoids maintained under newly established culture conditions. The human epidermal organoids showed an improved capacity of passaging for at least 10 rounds, enabling organoids to expand to cell numbers required for clinical applications. A newly designed auto micro-atomization device (AMAD) was developed for delivery of human epidermal organoids onto the sites of severe skin wounds enhancing uniform and concentrated delivery of organoids, facilitating their engraftment and differentiation for skin reconstitution. With the optimal design and using pneumatic AMAD, both survival and functions of organoids were effectively protected during the spraying process. Cells in the sprayed human epidermal organoids participated in the regeneration of the epidermis at wound sites in a mouse model and accelerated wound healing significantly. The novel AMAD and out new protocol with enhanced effects with respect to both organoid expansion and efficient transplantation will be used for clincal treatments of complex, uneven, or large-area severe skin wounds.
分类号一类
WOS关键词CELL DELIVERY ; BURNS ; KERATINOCYTES ; EPITHELIALIZATION ; FIBROBLASTS ; MULTICENTER ; EPITHELIUM
资助项目National Natural Science Foundations of China[81730052] ; Interdisciplinary Cooperation Project of Beijing Nova Program[Z1811100006218127] ; National Major Scientific and Technological Special Project for Significant New Drugs Development[2018ZX09711003-001-002] ; National Key Research and Development Program of China[2016YFC1101305]
WOS研究方向Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000523489100001
资助机构National Natural Science Foundations of China ; Interdisciplinary Cooperation Project of Beijing Nova Program ; National Major Scientific and Technological Special Project for Significant New Drugs Development ; National Key Research and Development Program of China
其他责任者Wang, Yunfang
源URL[http://dspace.imech.ac.cn/handle/311007/81819]  
专题力学研究所_非线性力学国家重点实验室
作者单位1.Univ N Carolina, Program Mol Biol & Biotechnol, Sch Med, Chapel Hill, NC 27515 USA
2.Univ N Carolina, Dept Cell Biol & Physiol, Sch Med, Chapel Hill, NC 27515 USA;
3.Chinese Peoples Liberat Army PLA, Dept Obstet & Gynecol, Air Force Med Ctr, Beijing, Peoples R China;
4.Chinese Peoples Liberat Army Gen Hosp, Inst TB Res, Army TB Prevent & Control Key Lab, Med Ctr 8, Beijing, Peoples R China;
5.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing, Peoples R China;
6.Tsinghua Univ, Beijing Tsinghua Changgung Hosp, Translat Res Ctr, Beijing, Peoples R China;
7.Inst Hlth Serv & Transfus Med, Stem Cell & Tissue Engn Lab, Beijing, Peoples R China;
推荐引用方式
GB/T 7714		 Chang MY,Liu J,Guo BL,et al. Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:16.
APA Chang MY.,Liu J.,Guo BL.,方新.,Wang Y.,...&Wang YF.(2020).Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,16.
MLA Chang MY,et al."Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):16.

入库方式： OAI收割

来源：力学研究所

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