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Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system

文献类型：期刊论文

作者Zhang, Sen1,2,3; Chen, Huan1; Wang, An2; Liu, Yong2; Hou, Hongwei1; Hu, Qingyuan1
刊名TOXICOLOGY MECHANISMS AND METHODS
出版日期2019-01-02
卷号29期号:1页码:1-7
ISSN号1537-6516
关键词gamma H2AX genotoxicity high content screening carbon monoxide 1,3-butadiene air-liquid interface system
DOI10.1080/15376516.2018.1477897
通讯作者Hou, Hongwei(qsfctc@163.com) ; Hu, Qingyuan(1965@163.com)
英文摘要To investigate the genotoxicity of gaseous toxicants carbon monoxide (CO) and 1,3-butadiene in vitro, a novel combination technology-the in vitro gamma H2AX high content screening assay combined with air-liquid interface system (ALIS) was established. The results showed that this new technology was available and effective. Based on the joint technology, genotoxicity of CO and 1,3-butadiene was evaluated further in this study. The results showed that treatment concentrations (0, 20%,40%, 80% and 100%, v/v) and exposure time (15, 30, 45, 60 and 90 min) of CO both had no statistically significant effects on the induction of gamma H2AX (p > 0.05). However, 1,3-butadiene can induce significant gamma H2AX (p < 0.01) in A549 cells in a dose/time-dependent manner both in the absence and presence of rat liver S9. When the concentrations of 1,3-butadiene were more than 80%, a higher gamma H2AX level could be induced than the 1.5-fold of vehicle controls after 1 h of treatment. Overall, this new technology can be used as a complementary tool to evaluate the genotoxicity of air-borne toxicants in vitro based on the in vitro gamma H2AX high content screening assay combined with ALIS. Based on the joint technology, CO was not genotoxic in A549 cells, while 1,3-butadiene showed significant genotoxicity in the dose/time-dependency on the induction of gamma H2AX.
WOS关键词CIGARETTE-SMOKE ; EXPOSURE ; MAINSTREAM ; TOXICITY ; IDENTIFICATION ; CYTOTOXICITY ; MUTAGENICITY ; BUTADIENE ; MODEL
资助项目National Natural Science Foundation of China[21347002]
WOS研究方向Toxicology
语种英语
出版者TAYLOR & FRANCIS LTD
WOS记录号WOS:000460459500001
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/42257]  
专题合肥物质科学研究院_应用技术研究所
通讯作者Hou, Hongwei; Hu, Qingyuan
作者单位1.China Natl Tobacco Qual Supervis & Test Ctr, Zhengzhou, Henan, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Appl Technol, Hefei, Anhui, Peoples R China
3.Univ Sci & Technol China, Hefei, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Sen,Chen, Huan,Wang, An,et al. Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system[J]. TOXICOLOGY MECHANISMS AND METHODS,2019,29(1):1-7.
APA Zhang, Sen,Chen, Huan,Wang, An,Liu, Yong,Hou, Hongwei,&Hu, Qingyuan.(2019).Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system.TOXICOLOGY MECHANISMS AND METHODS,29(1),1-7.
MLA Zhang, Sen,et al."Genotoxicity evaluation of carbon monoxide and 1,3-butadiene using a new joint technology: the in vitro gamma H2AX HCS assay combined with air-liquid interface system".TOXICOLOGY MECHANISMS AND METHODS 29.1(2019):1-7.

入库方式： OAI收割

来源：合肥物质科学研究院

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