中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability

文献类型:期刊论文

作者Wang, Zhaojun1,2; Cai, Yanan1; Qian, Jia1; Zhao, Tianyu1; Liang, Yansheng1; Dan, Dan1; Lei, Ming2; Yao, Baoli1
刊名Biomedical Optics Express
出版日期2020-06-01
卷号11期号:6页码:3058-3070
ISSN号21567085
DOI10.1364/BOE.391024
产权排序1
英文摘要

Super-resolution (SR) fluorescence microscopy that breaks through the diffraction barrier has drawn great interest in biomedical research. However, obtaining a high precision three-dimensional distribution of the specimen in a short time still remains a challenging task for existing techniques. In this paper, we propose a super-resolution fluorescence microscopy with axial localization capability by combining multifocal structured illumination microscopy with a hybrid detection PSF composed of a Gaussian PSF and a double-helix PSF. A modified reconstruction scheme is presented to accommodate the new hybrid PSF. This method can not only recover the lateral super-resolution image of the specimen but also retain the specimen's depth map within a range of 600 nm with an axial localization precision of 20.8 nm. The performance of this approach is verified by testing fluorescent beads and tubulin in 293-cells. The developed microscope is well suited for observing the precise 3D distribution of thin specimens. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

语种英语
出版者OSA - The Optical Society
源URL[http://ir.opt.ac.cn/handle/181661/93525]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Lei, Ming
作者单位1.State Key Laboratory of Transient Optics and Photonics, Xi' An Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi' an; 710119, China;
2.Shaanxi Key Laboratory of Quantum Information and Quantum Optoelectronic Devices, School of Science, Xi'An Jiaotong University, Shaanxi; 710049, China
推荐引用方式
GB/T 7714
Wang, Zhaojun,Cai, Yanan,Qian, Jia,et al. Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability[J]. Biomedical Optics Express,2020,11(6):3058-3070.
APA Wang, Zhaojun.,Cai, Yanan.,Qian, Jia.,Zhao, Tianyu.,Liang, Yansheng.,...&Yao, Baoli.(2020).Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability.Biomedical Optics Express,11(6),3058-3070.
MLA Wang, Zhaojun,et al."Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability".Biomedical Optics Express 11.6(2020):3058-3070.

入库方式: OAI收割

来源:西安光学精密机械研究所

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