Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability
文献类型:期刊论文
作者 | Wang, Zhaojun1,2; Cai, Yanan1; Qian, Jia1; Zhao, Tianyu1; Liang, Yansheng1; Dan, Dan1; Lei, Ming2; Yao, Baoli1 |
刊名 | Biomedical Optics Express |
出版日期 | 2020-06-01 |
卷号 | 11期号:6页码:3058-3070 |
ISSN号 | 21567085 |
DOI | 10.1364/BOE.391024 |
产权排序 | 1 |
英文摘要 | Super-resolution (SR) fluorescence microscopy that breaks through the diffraction barrier has drawn great interest in biomedical research. However, obtaining a high precision three-dimensional distribution of the specimen in a short time still remains a challenging task for existing techniques. In this paper, we propose a super-resolution fluorescence microscopy with axial localization capability by combining multifocal structured illumination microscopy with a hybrid detection PSF composed of a Gaussian PSF and a double-helix PSF. A modified reconstruction scheme is presented to accommodate the new hybrid PSF. This method can not only recover the lateral super-resolution image of the specimen but also retain the specimen's depth map within a range of 600 nm with an axial localization precision of 20.8 nm. The performance of this approach is verified by testing fluorescent beads and tubulin in 293-cells. The developed microscope is well suited for observing the precise 3D distribution of thin specimens. © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. |
语种 | 英语 |
出版者 | OSA - The Optical Society |
源URL | [http://ir.opt.ac.cn/handle/181661/93525] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Lei, Ming |
作者单位 | 1.State Key Laboratory of Transient Optics and Photonics, Xi' An Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi' an; 710119, China; 2.Shaanxi Key Laboratory of Quantum Information and Quantum Optoelectronic Devices, School of Science, Xi'An Jiaotong University, Shaanxi; 710049, China |
推荐引用方式 GB/T 7714 | Wang, Zhaojun,Cai, Yanan,Qian, Jia,et al. Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability[J]. Biomedical Optics Express,2020,11(6):3058-3070. |
APA | Wang, Zhaojun.,Cai, Yanan.,Qian, Jia.,Zhao, Tianyu.,Liang, Yansheng.,...&Yao, Baoli.(2020).Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability.Biomedical Optics Express,11(6),3058-3070. |
MLA | Wang, Zhaojun,et al."Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability".Biomedical Optics Express 11.6(2020):3058-3070. |
入库方式: OAI收割
来源:西安光学精密机械研究所
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