氨态氮对菲律宾蛤仔毒理机制研究的内参基因筛选
文献类型：期刊论文
| 作者 | 曹滕飞; 丛明 ; 李兆艳; 赵建民; 吕家森; 李成华
|
| 刊名 | 生态毒理学报
 |
| 出版日期 | 2017-04-15 |
| 卷号 | 12期号:2页码:182-190 |
| 关键词 | ammonia nitrogen Ruditapes philippinarum reference gene 氨氮 菲律宾蛤仔 内参基因 |
| ISSN号 | 1673-5897 |
| DOI | DOI: 10.7524/AJE.1673-5897.20160727001 |
| 其他题名 | Selection of Reference Genes for Toxicological Mechanism Research on Ruditapes philippinarum Exposed to Ammonia Nitrogen |
| 产权排序 | (1) 宁波大学海洋学院 ; (2) 中国科学院烟台海岸带研究所 ; (3) 烟台大学生命学院 |
| 文献子类 | Article |
| 英文摘要 | In order to analyze the transcriptome data accurately and systemically, a suitable reference gene is necessary. In the present study, geNorm and Normfinder softwares were used to analyze the expression stabilities of six candidate reference genes of Ruditapes philippinarum after ammonia nitrogen exposure for 30 days. The reference genes included 18S rRNA (18S), beta-actin (Actin), beta-tubulin (Tubu), elongation factor 1-alpha (EF1alpha), cyclophilin A (CyPA) and Ubiquitin (Ub). Based on a 50-times diluted cDNA in gills as the quantitative real-time PCR template, the geNorm software analysis demonstrated that the expression stabilities of the six candidate reference gene after NH_3-N exposure were as follows: EF1alpha>CyPA>Actin>Tubu>Ub>18S, and EF1alpha and CyPA were the best two ones among the six reference genes. A little different order was detected by the NormFinder software as follows: EF1alpha>Actin>Tubu>CyPA>Ub>18S. And EF1alpha was recommended as the optimal reference gene. As an assay, EF1alpha and CyPA were employed to examine one target unigene by qRT-PCR respectively. Final results showed that the variation trend detected by EF1alpha was more consistent with the foldchange value of the transcriptome. So EF1alpha was selected as the housekeeping gene to analyze the transcriptome data of gills in Ruditapes philippinarum after ammonia nitrogen exposure. |
| WOS关键词 | Environmental Sciences & Ecology |
| WOS研究方向 | Environmental Sciences & Ecology ; 环境科学与生态学 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6012838 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24244]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_污染过程与控制实验室 |
| 作者单位 | 1.中国科学院烟台海岸带研究所; 2.宁波大学海洋学院; 3.烟台大学生命学院 |
| 推荐引用方式 GB/T 7714 | 曹滕飞,丛明,李兆艳,等. 氨态氮对菲律宾蛤仔毒理机制研究的内参基因筛选[J]. 生态毒理学报,2017,12(2):182-190. |
| APA | 曹滕飞,丛明,李兆艳,赵建民,吕家森,&李成华.(2017).氨态氮对菲律宾蛤仔毒理机制研究的内参基因筛选.生态毒理学报,12(2),182-190. |
| MLA | 曹滕飞,et al."氨态氮对菲律宾蛤仔毒理机制研究的内参基因筛选".生态毒理学报 12.2(2017):182-190. |
入库方式： OAI收割
来源：烟台海岸带研究所
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