基于核磁共振的褐牙鲆幼鱼组织代谢组学分析
文献类型：期刊论文
| 作者 | 刘佳琳 ; 徐兰兰; 路珍; 吉成龙; 李斐; 王清; 赵建民; 吴惠丰
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| 刊名 | 海洋通报
 |
| 出版日期 | 2017-10-15 |
| 卷号 | 36期号:5页码:538-546 |
| 关键词 | Paralichthys olivaceus metabolomics NMR data preprocessing 褐牙鲆 代谢组学 核磁共振 数据预处理 |
| ISSN号 | 1001-6392 |
| DOI | Doi:10.11840/j.issn.1001-6392.2017.05.009 |
| 其他题名 | NMR-based metabolomic analysis on the extracts of whole tissue from flounder Paralichthys olivaceus |
| 产权排序 | (1) 中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室 ; (2) 中国科学院大学 |
| 文献子类 | Article |
| 英文摘要 | As an-omic technique in system biology, nuclear magnetic resonance(NMR) -based metabolomics has been widely used in marine biology in recent years.Due to the high salinity of marine environment, there exists substantial differences of metabolite abundances in marine organisms compared with other organisms.To deal with the high salinity of seawater, marine organisms use very high concentrations of osmolytes, such as taurine, betaine and trimethylamine N-oxide, to balance the osmolarity.Generally, the concentrations of osmolytes are hundreds of times higher than other metabolites, leading to extreme imbalance of weights of metabolites.Therefore, a suitable data preprocessing was the key procedure to accurately analyze the metabolomic data from marine organisms.In this work, the juvenile flounder Paralichthys olivaceus was exposed to a reduced salinity(50 % normal salinity, 15.6 psu).After exposure for 48 h, the one dimensional ~1H NMR spectroscopy was used to analyze the metabolomes of flounder samples.To investigate the influences of data preprocessing, the metabolomic data of founder samples were subjected to three different data preprocessing methods(unit variance scaling, Pareto scaling and generalized log transformation -mean centering) before pattern recognition analysis.Results indicated that the concentration of osmolyte, trimethylamine N-oxide, was approximately 200 times higher than other metabolites in flounder samples.The reduced salinity induced significant metabolic responses in juvenile flounder.After orthogonal partial least squares-discriminant analysis(OPLS-DA), generalized log transformation-mean centering was found to be the best data preprocessing method, presenting 8 significantly altered metabolites induced by reduced salinity, which was consistent with the quantitative analysis of metabolites.Overall, this study provided a suitable data preprocessing method to analyze the metabolomic data of flounder P.olivaceu. |
| WOS关键词 | Oceanography |
| WOS研究方向 | Oceanography ; 海洋学 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6096618 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24282]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_支撑部门 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室; 2.中国科学院大学 |
| 推荐引用方式 GB/T 7714 | 刘佳琳,徐兰兰,路珍,等. 基于核磁共振的褐牙鲆幼鱼组织代谢组学分析[J]. 海洋通报,2017,36(5):538-546. |
| APA | 刘佳琳.,徐兰兰.,路珍.,吉成龙.,李斐.,...&吴惠丰.(2017).基于核磁共振的褐牙鲆幼鱼组织代谢组学分析.海洋通报,36(5),538-546. |
| MLA | 刘佳琳,et al."基于核磁共振的褐牙鲆幼鱼组织代谢组学分析".海洋通报 36.5(2017):538-546. |
入库方式： OAI收割
来源：烟台海岸带研究所
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