石墨烯与抑癌基因p53 DNA片段相互作用的分子模拟与光谱学验证
文献类型：期刊论文
| 作者 | 吴惠丰 ; 张明兴; 李斐; 曹天贵; 李雪花; 赵建民
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| 刊名 | 生态毒理学报
 |
| 出版日期 | 2017-06-01 |
| 卷号 | 12期号:3页码:243-250 |
| 关键词 | p53-DNA graphene(G) p53-DNA binding interaction molecular dynamics simulations spectroscopic methods 石墨烯 相互作用 分子动力学模拟 光谱法 |
| ISSN号 | 1673-5897 |
| DOI | DOI:10.7524/AJE.1673-5897.20170104001 |
| 其他题名 | Studies on the Interaction between Graphene and p53-DNA by Molecular Dynamics Simulations and Spectroscopic Methods |
| 产权排序 | (1) 中国科学院烟台海岸带研究所中国科学院环境过程与生态修复重点实验室 ; (2) 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 ; (3) 中国科学院大学 ; (4) 大连理工大学环境学院工业生态与环境工程教育部重点实验室 |
| 文献子类 | Article |
| 英文摘要 | Graphene and its derivatives have been widely used in the fields ranging from energy to biomedicine because of its peculiar physical and chemical properties. However,limited attention has been paid to potential hazards of graphene to organisms and environments. In this study,the interaction between graphene and the promoter region of p53 gene (p53-DNA) was investigated using molecular dynamics simulation (MD) technology and spectroscopic methods based on the combination of UV-vis absorption,DNA melting point test and fluorescent spectra,followed by the illustration of the interaction mechanism. MD results showed that some aromatic moieties of graphene could intercalate into the p53-DNA base pairs,which relied on pi-pi stacking interaction between aromatic moieties of graphene and p53-DNA base,and the other parts of graphene could further combine with p53-DNA by groove binding. Moreover,spectroscopic tests exhibited that graphene could increase the melting point (T_m) of p53-DNA and lead to static fluorescence quenching of EB-DNA system,suggesting that graphene was indeed able to bind with p53-DNA. Meanwhile,the ascent of absorbance of 260 nm was found in the p53-DNA as a result of the combination of graphene and p53-DNA,which meant graphene posed some effect on the double helix structure of p53-DNA. In conclusion,the mechanism of interaction between graphene and p53-DNA was investigated at molecular level in this study,which contributed to a further understanding of the toxicological effects of graphene. |
| WOS关键词 | Environmental Sciences & Ecology |
| WOS研究方向 | Environmental Sciences & Ecology ; 环境科学与生态学 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6082095 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24294]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_污染过程与控制实验室 烟台海岸带研究所_支撑部门 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 中国科学院烟台海岸带研究所 |
| 推荐引用方式 GB/T 7714 | 吴惠丰,张明兴,李斐,等. 石墨烯与抑癌基因p53 DNA片段相互作用的分子模拟与光谱学验证[J]. 生态毒理学报,2017,12(3):243-250. |
| APA | 吴惠丰,张明兴,李斐,曹天贵,李雪花,&赵建民.(2017).石墨烯与抑癌基因p53 DNA片段相互作用的分子模拟与光谱学验证.生态毒理学报,12(3),243-250. |
| MLA | 吴惠丰,et al."石墨烯与抑癌基因p53 DNA片段相互作用的分子模拟与光谱学验证".生态毒理学报 12.3(2017):243-250. |
入库方式： OAI收割
来源：烟台海岸带研究所
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