紫金牛叶杆菌RC6b 及其诱变菌株对二苯砷酸的降解特征
文献类型:期刊论文
作者 | 宋芳; 涂晨![]() ![]() |
刊名 | 微生物学通报
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出版日期 | 2017-06-06 |
卷号 | 44期号:11页码:2557-2566 |
关键词 | Diphenylarsinic acid Phyllobacterium myrsinacearum Co-metabolic degradation Chemical mutagenesis Metabolites 二苯砷酸 紫金牛叶杆菌 共代谢降解 化学诱变 代谢产物 |
ISSN号 | 0253-2654 |
DOI | DOI: 10.13344/j.microbiol.china.170041 |
其他题名 | Diphenylarsinic acid degradation by Phyllobacterium myrsinacearum RC6b and its mutants |
产权排序 | (1) 中国科学院海岸带环境过程与生态修复重点实验室烟台海岸带研究所 ; (2) 中国科学院大学 |
文献子类 | Article |
英文摘要 | [Objective] To clarify the ability of Phyllobacterium myrsinacearum RC6b and its mutant strains to degrade diphenylarsinic acid (DPAA). [Methods] The conditions for the co-metabolic degradation of DPAA were optimized with P. myrsinacearum RC6b as the original strain and with sucrose, glucose and sodium acetate as different external carbon sources. Strain RC6b was chemically mutated using treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The DPAA degradation rate was compared before and after chemical mutagenesis, and the metabolite of DPAA by RC6b mutants was also analyzed. [Results] The DPAA degradation rate was <2% after 28 days of incubation when using DPAA as the sole carbon source. After addition of sucrose, glucose and sodium acetate as external carbon sources the DPAA degradation rate increased significantly, by 14.08%, 15.21% and 15.05%, respectively. Three mutant strains of strain RC6b were obtained using 250 mug/mL NTG as chemical mutagen. All three RC6b mutants showed significantly higher DPAA degradation rates than did the original strain. Mutant strain N-RC6b2 showed the highest DPAA degradation rate of 36.71% after 28 days of incubation when using DPAA as the sole carbon source. The main degradation metabolite of DPAA by strain N-RC6b2 was identified as monohydroxylated DPAA. [Conclusion] The original RC6b strain grew only sparingly using DPAA as the sole carbon source. Addition of co-metabolic carbon sources such as sucrose, glucose and sodium acetate significantly enhanced the biodegradation of DPAA. The chemical mutagen NTG further enhanced the biodegradation of DPAA, with monohydroxylated DPAA as the main intermediate product. |
WOS关键词 | Environmental Sciences & Ecology |
WOS研究方向 | Environmental Sciences & Ecology ; 环境科学与生态学 |
语种 | 中文 |
CSCD记录号 | CSCD:6109404 |
源URL | [http://ir.yic.ac.cn/handle/133337/24308] ![]() |
专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_污染过程与控制实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.中国科学院海岸带环境过程与生态修复重点实验室烟台海岸带研究所; 2.中国科学院大学 |
推荐引用方式 GB/T 7714 | 宋芳,涂晨,朱濛,等. 紫金牛叶杆菌RC6b 及其诱变菌株对二苯砷酸的降解特征[J]. 微生物学通报,2017,44(11):2557-2566. |
APA | 宋芳,涂晨,朱濛,刘颖,&骆永明.(2017).紫金牛叶杆菌RC6b 及其诱变菌株对二苯砷酸的降解特征.微生物学通报,44(11),2557-2566. |
MLA | 宋芳,et al."紫金牛叶杆菌RC6b 及其诱变菌株对二苯砷酸的降解特征".微生物学通报 44.11(2017):2557-2566. |
入库方式: OAI收割
来源:烟台海岸带研究所
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